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3
Gout Examination Procedure
Slider insertion direction
1. Engage the 40X objective in the light path.
2. If the condenser in use is equipped with a top lens, engage it
in the light path.
3. Set the
-plate rotation lever of the U-GAN to the center posi-
tion ( · ). Look into the eyepiece and rotate the polarizer to the
darkest position.
4. Rotate the stage or specimen so that the longitudinal direction
of the crystals is in parallel with the -axis @.
At this time, the -axis direction of the
-plate should be iden-
tical to the longitudinal direction of crystals.
5. Rotate the
-plate rotation lever of the U-GAN fully counter-
clockwise (
) and diagnose the gout.
Crystals are yellow Sodium urate (Gout crystal)
Crystals are blue Potassium pyrophosphate (Pseudogout crystal)
6. Rotate the -plate rotation lever of the U-GAN fully clock-
wise ( ) so that the longitudinal direction of crystal is
perpendicular to the -axis ². The examination result can be
confirmed by referring to the reversal of the color.
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Other Observations
Brightfield Observation
Loosen the clamping knob of the revolving nosepiece clamping the U-GAN, pull out the U-GAN and stop at a click
position. (Tighten the clamping knob there.)
Now the -plate is disengaged from the light path so brightfield observation is available.
Other
Set the
-plate rotation lever of the U-GAN to the center position ( · ) for simple polarized light observation. However, the
contrast of the observed image may be poor because of the presence of the
-plate.
Crystal
(Perpendicular to -axis ²)
(Parallel with -axis @)
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