Palm MicroBeam User Manual (English)

Donald WuAug 3, 2025

How to fix Palm MicroBeam when the laser is not cutting?

A
amy78Aug 3, 2025
If the laser is not cutting, you should: * Check energy and focus settings as described in the user manual, if necessary, take factory settings. * Check the red and green LEDs on the laser unit. If the red or green LED is off, check the key switch on the laser unit or the function of the safety switch in the illumination arm of the microscope, respectively. * Ensure the shutter is open. If the shutter is closed, open it. * Confirm that the filter revolver is switched to 'laser' (unless you have fluorescence equipment with P.A.L.M. simultaneous cutting). * Also, verify the position of the adjusting aid for the fluorescence lamp, the shutter for FL, both sliders with diaphragm, the filter slider, and the correction ring of the objective according to the thickness of the slide.

Brand	Palm
Model	MicroBeam
Category	Laboratory Equipment
Language	English

Brand

Palm

Model

MicroBeam

Important Basic Information

Legal Notes

Legal disclaimers regarding liability and warranty conditions for the user manual.

Conventions for this User Manual

Explains special markings and symbols used in the manual for user guidance.

How You Can Contact Us

Provides contact details for P.A.L.M. Microlaser Technologies AG, including phone, fax, email, and address.

Operating Room

Admissible Ambient Conditions

Specifies the recommended ambient temperature and humidity for operating the PALM® MicroBeam system.

Safety

Emergency Procedures

Outlines immediate steps to take in case of an emergency with the device.

User Manual Adherence

Emphasizes the importance of reading and following the user manual for safe operation and device longevity.

Laser Safety

Details safety requirements for operating the Class 1M/3B laser device, including precautions against direct exposure.

Safety Markings and Type Plate

Explains the importance of legible safety markings and the device's type plate for user safety.

Staff Qualification

Specifies requirements for trained and instructed personnel to operate the device safely.

General Safety Points

Lists critical safety points and general precautions for operating and maintaining the device.

Laser, Electrical, and Infectious Risks

Details risks associated with laser, electricity, and infectious materials, along with protective measures.

EMV and Fire Extinction Risks

Covers EMV risks and fire extinction guidelines, specifying suitable agents and warning against water use.

Safe and Ecological Disposal

Offers environmentally friendly disposal methods for various system components and materials.

The PALM® MicroBeam - Survey

PALM® ControlUnit

Describes the main device that runs and controls the system components like RoboStage, energy, and focus units.

Laser Interface

Explains the one-box device housing the laser and optics to guide and focus the beam into the microscope.

Laser

Details the pulsed UV laser (337nm) used, its beam quality, and factors affecting cutting width.

Microscope Adapter

Describes the adapter that connects the laser to the microscope, guiding the beam into the objective.

Microscope Zeiss Axiovert 200

Covers the high-quality Zeiss microscope integrated into the PALM® MicroBeam system.

Fluorescence Attachment

Explains the epifluorescence attachment that expands the microscope for fluorescence microscopy.

PALM® RoboStage

Details the computer-controlled motorized specimen stage designed for high-precision laser micromanipulation.

PALM® CapMover

Describes the motorized device for fast positioning and quick cap exchange for sample collection.

Control Apparatus

Details the control apparatus for the CapMover, including buttons, joystick, and LED indicators.

Mechanical Manipulator

Explains the alternative mechanical manipulator for cap movement via hand-driven knobs.

CCD Video Camera

Covers the CCD camera for transmitting microscope images to the computer for real-time observation and storage.

Computer

Describes the state-of-the-art computer system with image handling software and network connectivity.

How to Prepare the PALM® MicroBeam for the Application

Switch On/Off the Device

Provides instructions for powering the system on and off, including ControlUnit and computer.

CCD Video Camera Adapter

Mentions the camera adapter and calibration necessity, referring to the RoboSoftware Manual.

Laser Check in Microscope

Details steps to check the laser coupling, aperture, filters, and shutter status in the microscope.

Laser Energy and Focus

Explains how to set laser focus and energy for precise cutting and catapulting.

Adjust Laser Focus

Provides methods for adjusting laser focus using graphical tools and observing ablation effects.

Cutting Speed

Discusses optimizing cutting speed and laser energy for fine cutting lines and large area microdissection.

Trouble Shooting List

Laser is not cutting

Provides a checklist to diagnose and resolve issues when the laser is not cutting effectively.

No video image

Offers troubleshooting steps for issues related to the absence of a video image from the camera.

Shift between drawing and cutting

Guides on resolving problems with switching between drawing mode and cutting mode.

Maintenance of the PALM® MicroBeam

Laser Maintenance

Refers to a separate Laser Manual for detailed maintenance procedures for the laser component.

Microscope Maintenance

Provides guidance for microscope maintenance, referring to the Microscope Manual.

Cleaning Optical Parts

Provides instructions for cleaning accessible optical parts of the microscope using alcohol.

Cleaning When Dirty

Details methods for cleaning soiled optical parts, emphasizing prompt removal of dirt to prevent permanent impairment.

Technical Data

Electricity

Lists technical specifications related to the electrical input voltage, frequency, and fluctuations.

Nitrogen Laser

Provides technical data for the nitrogen laser, including wavelength, pulse energy, duration, and divergence.

Palm MicroBeam User Manual

Table of Contents

Questions and Answers:

Palm MicroBeam Specifications

Summary