Sorvall RT/T6000D - Page 204

RT6000B
and
T6000B
Centrifuges
SORV
ALL®
Centrifuges
Serial Numbers Below
9101800
2.
Install
an
H- 1000B
swinging
bucket
rotor
onto
the
drive
spindle.
Be
sure
that
all
bucket
trunnions
are
cleaned
and
well
lubricated
and
that
all
four
buckets
swing
freely
on
rotor
trunnions.
Place
tube
carriers
into
rotor
buckets.
3.
Remove two
opposing
buckets
from
rotor,
with
carriers installed, and
place
onto
a
scale.
4. Prepare
three
(3)
test tubes
of
varying
levels
of
water:
a.
Fill
one
tube
full
of water
and
mark
the tube
with
a
"O".
Place the
tube,
filled
with water
into
one
bucket
carrier.
This
tube
will
be used
as a
"reference"
tube.
b.
Fill
a
second
tube with
less water
than the
"reference"
tube and
place
into
the
opposite
bucket
carrier
so
that
the
net
difference
in
weight between
the two
buckets
equal
19
grams.
Mark
the
second
tube
with
a
"19".
This tube
will be
used
as
the
"pass"
tube.
c.
Fill
a
third
tube with two (2)
grams
more
water than the
"pass"
tube.
Mark
the
third
tube
with
a
"21".
This
tube will be
used
as
the
'Jail"
tube.
5.
Place
both
buckets and
carriers
back
onto
the
rotor in
the
chamber.
6.
Place the
"reference"
tube into
one
bucket
carrier and the
"fail"
tube
in
the
opposite
bucket
carrier.
Close
and
lock the
door.
NOTE
To
ensure
that the
circuit
can
be
calibrated,
the
gap
setting must be
pre¬
tested.
Follow
steps
7
through
16
to
pre-test
the
gap
setting,
then
continue
the
calibration
with
step
17.
7.
Adjust
R-3
to
read
+4.0
Vdc
at
I.C.
U4
pin
4.
The
+4.0
Vdc
should
cause
the
imbalance
circuit to
"trip"
with the
"fail"
tube installed.
8.
Set
the
speed
control
knob to
3200
rpm
setting.
9.
Set
the
timer
control knob to
the
"HOLD"
position
to
start
the
centrifuge.
The
rotor
should
begin accelerating
towards
3200
rpm.
NOTE
The
FAULT LED
indicator
on
the
front
console
should
illuminate
as
the
rotor
accelerates between
500
rpm
and
1000
rpm.
10.
If
the
rotor
speed
exceeds
1500
rpm,
stop
the
run
in
progress by
turning
the
speed
control knob
to
off.
If
the
circuit
did
not
"trip"
at
the
+4.0
Vdc
limit, remove
the
rotor and
regap
the
set screw
by
turning
it
1/8
turn
clockwise. Retest
the
gap
setting
again
and
continue adjusting
the
gap
until
the rotor
trips
at
+4.0 Vdc.
8-34
Rev.
7/93