EasyManua.ls Logo

Thermo Scientific HERACELL VIOS 160i LK User Manual

Thermo Scientific HERACELL VIOS 160i LK
234 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
Page #1 background imageLoading...
Page #1 background image
50162755 A July 2022
Thermo Scientific
HERACELL VIOS 160i LK/
HERACELL VIOS 250i LK
CO
2
Incubator
User Manual

Table of Contents

Question and Answer IconNeed help?

Do you have a question about the Thermo Scientific HERACELL VIOS 160i LK and is the answer not in the manual?

Thermo Scientific HERACELL VIOS 160i LK Specifications

General IconGeneral
TypeCO2 Incubator
Temperature RangeAmbient +3°C to 55°C
Temperature Uniformity±0.3°C at 37°C
CO2 Control Range0 to 20%
CO2 Control Accuracy±0.1%
O2 Control Range1 to 21%
Capacity165 liters
Humidity RangeUp to 95% RH
Sterilization180°C
Shelves4 standard
Electrical Requirements230V, 50/60Hz

Summary

Preface

General Notes

Provides an overview of general information, device identification, and operating personnel instructions.

Applicability of the Instructions

Outlines how the operating instructions apply and notes regarding translations and accessibility.

Warranty

Specifies the conditions and duration for the incubator's operational warranty.

Explanation of Safety Information and Symbols

Defines warning, caution, and notice symbols used in the operating instructions for safety awareness.

Intended Purpose of the Device

Defines the correct use and application scope of the CO2 incubator.

Safety Notes on Gases

Provides critical safety information regarding the use of gases like CO2, O2, and N2 with the incubator.

Delivery of the Incubator

Acceptance Inspection

Instructs on immediate checks upon delivery for completeness and any potential damage.

Standard Equipment Components HERACELL VIOS 160i LK / 250i LK

Lists all components included as standard with the HERACELL VIOS 160i LK / 250i LK models.

Optional Equipment Components HERACELL VIOS 160i LK

Lists optional components available for the HERACELL VIOS 160i LK model.

Optional Equipment Components HERACELL VIOS 250i LK

Lists optional components available for the HERACELL VIOS 250i LK model.

Installation of the Device

Environmental Conditions

Specifies the required ambient conditions for operating the device safely and effectively.

Space Requirements

Outlines the necessary clearances and accessibility requirements for device installation.

Transport

Provides instructions and warnings for safely transporting the device, emphasizing correct lift points.

Stacking

Explains the possibility and methods for stacking multiple devices, including the use of adapters.

Installing the Support Stand with Castors

Provides step-by-step instructions for installing the support stand with castors.

Retrofitting/Modifications

Gas-Tight Screens

Options include threefold or sixfold screens, with or without Cell Locker, for improved sample access.

Infrared (IR) CO2 Sensor

Optional sensor for enhanced CO2 measurement accuracy and performance.

4-20 mA Data Interface

Optional interface for data output, converting internal values to a continuous output current.

Gas Cylinder Monitor

Optional system for monitoring gas supply levels and providing supply status information.

Description of the Device

HERACELL VIOS 160i LK / VIOS 250i LK Front View

Illustrates and identifies components visible on the front of the HERACELL VIOS 160i LK and 250i LK models.

HERACELL VIOS 160i LK / VIOS 250i LK Rear View

Shows and labels the components and connections located on the rear of the incubator.

Safety Devices HERACELL VIOS 160i LK / VIOS 250i LK

Details the built-in safety features designed to protect the user and the samples.

Workspace Atmosphere

Explains the controlled environmental conditions within the incubator's workspace.

Water Quality Recommendations

Provides guidelines on the type and quality of water required for the humidity reservoir to ensure optimal performance.

HEPA-Filter and Air Duct

Explains the role of the HEPA-filter in minimizing contamination and the function of the air duct for temperature distribution.

CO2 Supply

Details the CO2 supply requirements, control range, and gas quality specifications for cell culture growth.

Sensor System

Details the various sensors installed for monitoring temperature, O2, CO2, and humidity within the workspace.

Supply Interface

Standard Interfaces

Illustrates and lists the standard connectors on the control box for basic functions and optional elements.

Optional Interfaces

Lists interfaces available only with specific optional configurations, such as 4-20 mA and O2 connectors.

Gas Connection

Explains how to connect gas supply lines, required pressure, and the function of the gas inlet filter.

USB Interface

Details the USB interface for PC connection, data acquisition, and driver software requirements.

Alarm Contact

Describes how to connect the device to an external alarm system via a potential-free alarm contact.

Power Supply Connection

Guides on connecting the unit to the power supply, including safety checks for voltage and cable integrity.

Workspace Components

Interior Container

Describes the design of the interior container for preventing contamination and ensuring air quality.

Glass Door and Optional Gas-Tight Screen

Describes the standard glass door and the benefits of optional gas-tight screens for reduced contamination.

Water Reservoir

Details the water reservoir's design, separation from the workspace, and draining mechanism.

Water Filling

Guides the user on how to fill the water reservoir using a re-filling funnel and the correct water levels.

Shelf System

Details the perforated support rails and shelves, including tilt protection and withdrawal stops.

Electromechanical Door Lock Assembly

Explains the function of the door lock assembly as a safety device during decontamination routines.

Start-up

Preparing the Workspace

Outlines the necessary cleaning of workspace components before initial start-up and decontamination.

Installing the “MAX” Fill Level Indicator and the Pre-Filter

Provides instructions for installing the fill level indicator and pre-filter without tools.

Install the Air Duct

Details the assembly process for the air duct, including joining parts and locking keyholes.

Installing the HEPA-Filter and the Water Reservoir Cover Panel

Provides steps for inserting the HEPA-filter into the airbox and placing the airbox onto the cover panel.

Installation/Removal of the Support Rail

Explains how to install and remove support rails for the shelving system using spring pressure.

Installing the Shelves

Details the process of sliding shelves onto supports, including tilt protection and withdrawal stops.

Mount the Split Inserts (Optional) into the HERACELL VIOS 250i LK

Guides the installation of optional split inserts into the HERACELL VIOS 250i LK for customized shelving.

Installing Gas Pressure Hoses

Provides instructions for connecting gas pressure hoses, including safety precautions for pressure compensation.

Combined CO2 and O2/N2 Connection with Gas Monitoring System (Optional)

Explains how to connect combined CO2/O2/N2 supply with an optional gas monitoring system.

Power Supply Connection

Guides on connecting the unit to the power supply, including safety checks for voltage and cable integrity.

Connecting the USB Interface

Details how to connect the unit via USB, including driver installation and baud rate settings.

Connecting the Alarm Contact

Explains how to connect the device to an external alarm system using the potential-free contact.

Connecting the 4-20 mA Interface

Shows the pin-out for the 4-20 mA data interface and notes on skilled work for connection.

Operation

Preparing the Device

Outlines pre-operation checks on gas hoses, access port, door seal, and other components.

Decontaminating the Device Workspace

Instructs on running the steri-run routine or other decontamination methods for the workspace.

Starting Operation

Provides a step-by-step guide to starting the incubator, including opening doors and filling the water reservoir.

Starting the Device

Details the steps for initiating the device with the auto-start routine and setting parameters.

Loading the Device

Provides guidance on loading the workspace with cultures, emphasizing air circulation and loading surface limits.

Handling and Control

Operating Panel and Operating Structure

Explains the touchscreen interface, its areas, and the function of various keys and icons.

Setting the Temperature Set Value

Guides on setting the incubator's temperature set value through the temperature menu.

Setting the CO2 Set Value

Guides on setting the CO2 concentration value through the CO2 menu.

Setting the O2 Set Value

Guides on setting the O2 concentration value, applicable to models with optional O2/N2 control.

Auto-Start Function

Details the automated routine for startup and adjustment of CO2 sensing circuits, including temperature and humidity stabilization.

Activating the Auto-Start Routine

Provides instructions for preparing and launching the auto-start routine, including gas supply and water level checks.

Interrupting the Auto-Start Routine

Explains how to interrupt, resume, or cancel the auto-start routine using the status dialog box.

Launching Steri-Run

Introduces the steri-run routine for decontaminating the workspace, shelf system, and sensors.

User Configuration

Allows user interface and device function adjustments to meet operational requirements.

Settings / Setup

Allows access to dialog windows for customizing the user interface, including keypad lock, date/time, and display settings.

Error Messages

Describes the error detection system, signals, and messages, including audible alarms and display indicators.

Resetting Overtemperature Protection

Explains how to display the cause of an overtemperature error and reset the protection.

Measures After Power Outage

Provides steps to take after a power outage to ensure safe operation and dry sensors, including disinfection if needed.

Troubleshooting

Presents error tables indicating failure messages, causes, remedies, and alarm status for system and temperature control loops.

Shut-Down

Shutting the Device Down

Provides a step-by-step procedure for safely shutting down the device, including decontamination and draining.

Cleaning and Disinfection

Cleaning

Offers safety hints and procedures for cleaning exterior surfaces and the display using appropriate agents.

Decontamination Procedures

Outlines compatible disinfection procedures like wipe/spray and the automated steri-run routine.

Wipe/Spray Disinfection

Describes the three stages of manual wipe/spray disinfection, including precautions for disinfectants and rinsing steps.

Steri-Run Decontamination Routine

Explains the automated steri-run cycle, its temperature, duration, germ reduction efficiency, and door lock mechanism.

Activating Steri-Run

Guides on activating the steri-run routine by pressing the key and starting the process.

Opening the Door after Canceling Steri-Run

Explains how to release the door lock after canceling steri-run or if it aborted due to an error, using the emergency release lever.

Maintenance

Inspections and Checks

Lists daily and annual checks required to ensure operational performance and safety of the device.

Service Intervals

Outlines the recommended service tasks to be performed at 3-month, 6-fold gas tight screen, and annual intervals.

Temperature Calibration Procedure

Provides a step-by-step guide for calibrating the incubator's temperature sensor using a measuring instrument.

CO2 Calibration Procedure

Guides on performing CO2 calibration by entering measured values into the calibration menu.

Replacing the HEPA-Filter

Provides step-by-step instructions for replacing the HEPA-filter, including powering down and removing components.

Replacing the Gas Inlet Filter

Explains how to replace the gas inlet filter, including shutting off gas supply and loosening the hose clamp.

Waste Disposal

Overview of the Materials Used

Lists the materials used in the device construction and their disposal guidelines.

Specifications

HERACELL VIOS 160i LK

Lists the detailed technical specifications for the HERACELL VIOS 160i LK model.

HERACELL VIOS 250i LK

Lists the detailed technical specifications for the HERACELL VIOS 250i LK model.

Data Communication

USB Interface

Describes the USB interface for PC connection, including compliance with standards and virtual COM port operation.

Installing the USB Port Driver

Guides on installing the USB driver software from the data CD using the Windows Hardware Wizard.

Structure of the Command Sequences for Data Communication

Explains the ASCII character-based command sequences for data exchange between PC and incubator.

Error Memory Structure

Explains the structure of the error memory, including possible error messages in hex and bit coding for control loops.

Data Logger Structure

Explains the data logger's storage capacity, information stored, and querying commands for accessing logged data.

Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK

Introduces the software program for managing data communication, including error logging and service file creation.

Operating the HERACELL VIOS 160i LK & HERACELL VIOS 250i LK

Details the operation of the user interface, including MAIN menu functions and GENERAL submenus like PRESETTING and ERROR LOGGER.

Device Log

Device Log

Provides a template for listing carried-out works, including device type, serial number, location, and details of maintenance performed.

Contact Information Thermo Scientific

Germany Contact Information

Contact details for sales, service, and helpdesk for Germany.

Europe, Middle East, and Africa Contact Information

Contact details for sales and service for Europe, Middle East, and Africa.

North America Contact Information

Contact details for sales and service inquiries in North America.

Latin America Contact Information

Contact details for sales and service inquiries in Latin America.

Asia Pacific Contact Information

Contact details for sales and service inquiries in the Asia Pacific region.

Other Regional Contacts

Lists contact details for USA/Canada, Australia/New Zealand, and other listed European countries.

Related product manuals