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Thermo Scientific Microm HM 430 User Manual

Thermo Scientific Microm HM 430
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Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 - English
1
Thermo Scientific
Sliding Microtome
Microm HM 430
Instruction Manual
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Thermo Scientific Microm HM 430 Specifications

General IconGeneral
Microtome TypeRotary Microtome
Section Thickness Settings0.5 µm increments
Trimming Thickness Settings1 µm increments
Vertical Specimen Stroke60 mm
ApplicationHistology, Pathology
Section Thickness Range0.5 - 60 µm
Trimming Thickness Range1 - 60 µm
Specimen OrientationHorizontal
Specimen IlluminationLED
Blade TypeDisposable blades
Power Supply230 V / 50 Hz or 115 V / 60 Hz

Summary

PRODUCT CERTIFICATION

PRODUCT WARRANTY

INTENDED USE OF MICROTOME

GENERAL SAFETY PRECAUTIONS

WARNING SIGNALS AND SYMBOLS EXPLANATION

Explains symbols like Note, Warning, Caution, Hand Injuries, Biohazard, Radioactivity.

ENVIRONMENTAL AND DISPOSAL GUIDELINES (WEEE)

Instructions for separate collection of electrical/electronic instruments.

OPERATOR SAFETY ATTENTION!

MICROTOME KNIFE HANDLING SAFETY

Safety instructions for handling and using the microtome knife.

EXPLOSIVE ENVIRONMENT HAZARD WARNING

Warnings against operating the instrument in explosive environments.

RADIOACTIVE RADIATION HAZARD

Safety procedures for working with radioactive specimens.

INFECTIOUS HAZARD WARNING

INTRODUCTION TO THE MICROTOME

MICROTOME HM 430 DESCRIPTION

Details the features and applications of the HM 430 microtome.

MICROTOME HM 430 TECHNICAL DATA

OPERATING INSTRUCTIONS

MICROTOME SETUP PROCEDURE

Instructions for unpacking and positioning the microtome for operation.

MICROTOME CUTTING MOVEMENT

SLEDGE LOCKING MECHANISM

SLEDGE ADDITIONAL BALLAST ASSEMBLY

SPECIMEN FEED MECHANISM

SETTING MICROTOME SECTION THICKNESS

How to set the desired section thickness using the scaled wheel.

MICROTOME COARSE FEED OPERATION

MANUAL FINE FEED OPERATION

AUTOMATIC FINE FEED OPERATION

AUTOMATIC FEED POSITION ADJUSTMENT

SPECIMEN CLAMPING SYSTEMS

STANDARD SPECIMEN CLAMP (TYPE 1)

How to use the standard specimen clamp for rectangular/square blocks.

STANDARD SPECIMEN CLAMP (TYPE 2)

Instructions for a second standard specimen clamp with transverse clamping.

UNIVERSAL CASSETTE CLAMP ASSEMBLY

SPECIMEN CLAMP REPLACEMENT

KNIFE CARRIER ASSEMBLY

KNIFE CLEARANCE ANGLE ADJUSTMENT

How to adjust the clearance angle of the knife using screws.

KNIFE GUARD PROTECTION AGAINST INJURY

SIDEWAYS KNIFE MOVEMENT

DIAGONAL KNIFE POSITIONING

OPTIONAL HEIGHT PLATES

MICROTOME KNIFE PROFILES

DISPOSABLE BLADE HOLDER OPERATION

BLADE INSERTION AND CLAMPING

Steps for inserting and clamping disposable blades into the holder.

DISPOSABLE BLADE CLEARANCE ANGLE

How to adjust the clearance angle for disposable blades.

KNIFE GUARD SAFETY DURING ADJUSTMENT

Importance of using the knife guard for safety during adjustments.

STANDARD MICROTOME EQUIPMENT

OPTIONAL EQUIPMENT: BLADE HOLDERS & CLAMPS

OPTIONAL DISPOSABLE BLADE HOLDERS

Catalog numbers for different disposable blade holders.

OPTIONAL SPECIMEN CLAMPS

Catalog numbers for universal and standard specimen clamps.

ADDITIONAL OPTIONAL ACCESSORIES

OPTIONAL HEIGHT PLATES

Catalog numbers for optional height adjustment plates.

OPTIONAL MICROTOME KNIVES

Catalog numbers for various steel and tungsten carbide microtome knives.

OPTIONAL KNIFE CASES

Catalog numbers for knife cases.

OPTIONAL PARAFFIN DISPOSABLE BLADES

Catalog numbers for disposable paraffin blades.

OPTIONAL ADDITIONAL ACCESSORIES

Catalog numbers for fast freezing unit and paraffin repellent.

THEORY OF MICROTOME OPERATION

MICROTOME CUTTING MOVEMENT PRINCIPLES

Explains the microtome's cutting mechanism using linear roller bearings.

THEORY: SPECIMEN COARSE FEED

THEORY: SPECIMEN CLAMPING & ORIENTATION

THEORY: KNIFE CARRIER FUNCTIONALITY

THEORY: FAST FREEZING UNIT

WORKING WITH THE MICROTOME

SPECIMEN PREPARATION & ORIENTATION FOR USE

Preparing and orienting specimens before sectioning.

COARSE FEED AND TRIMMING

Using coarse feed/trimming to adjust space between specimen and edge.

SECTIONING BEST PRACTICES

KNIFE EDGE SHARPNESS AND CONDITION

Importance of using a sharp knife and how to manage blunt edges.

CLEANING KNIFE SURFACES FOR SECTIONING

Importance of clean knife surfaces for optimal sectioning.

MICROTOME MALFUNCTION AVOIDANCE

SPECIMEN PREPARATION FOR MALFUNCTION AVOIDANCE

Ensuring proper specimen preparation, embedding, and fixation.

SPECIMEN TEMPERATURE EFFECTS ON PARAFFIN

Maintaining correct specimen temperature to avoid paraffin softening.

PROPER TIGHTENING OF CLAMPING SCREWS

Ensuring all clamping screws and levers are properly tightened.

GUIDELINES FOR KNIFE SELECTION

Importance of selecting the correct knife material and profile.

PROCEDURE FOR KNIFE CLEARANCE ADJUSTMENT

Proper adjustment of the knife's clearance angle.

GUIDELINES FOR CUTTING SPEED SELECTION

Selecting the appropriate cutting speed based on material hardness.

TRIMMING SPECIMENS WITH THE MICROTOME

Care required when bringing the knife and specimen together for trimming.

MICROTOME MAINTENANCE

ANNUAL ROUTINE MICROTOME MAINTENANCE

Recommendation for annual routine maintenance by a trained technician.

THERMO SCIENTIFIC SERVICE CONTRACTS

Information on Thermo Scientific service contracts for instrument maintenance.

MICROTOME CLEANING AND CARE

CLEANING INTERVALS AND AGENTS GUIDANCE

Factors influencing cleaning frequency and suitable cleaning agents.

MICROTOME CARE AND CLEANING PROCEDURES

Steps for cleaning the microtome, knife, and components.

INSTRUMENT TRANSPORTATION AND RETURN

RETURNING INSTRUMENT FOR REPAIR OR MAINTENANCE

Procedures for returning the instrument for service, including packaging.

INSTRUMENT CLOSING DOWN FOR TRANSPORT

Steps for closing down the instrument before transportation.

TRANSPORTATION MEASURES OUTSIDE BUILDINGS

INSTRUMENT DISPOSAL AND RECYCLING

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