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Thermo Scientific Microm HM355S User Manual

Thermo Scientific Microm HM355S
72 pages
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Thermo Fisher Scientific
Operation Manual HM335S
1
Part of Thermo Fisher Scientific
Microm HM355S
Rotary Microtome
Operation Manual – English / German
ex Ser. No 44478

Table of Contents

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Thermo Scientific Microm HM355S Specifications

General IconGeneral
Microtome TypeRotary
Feed MechanismMotorized
Horizontal specimen feed28 mm
Vertical stroke70 mm
Frequency50/60 Hz
Blade TypeDisposable blade
Specimen orientation8° X/Y
Specimen IlluminationOptional
Specimen ClampUniversal
Operating voltage100 – 240 V
Section Thickness Range0.5 to 100 µm
Trimming thickness range1 to 600 µm
Trimming thickness setting1 – 10 µm in 1 µm increments, 10 – 20 µm in 2 µm increments, 20 – 50 µm in 5 µm increments, 50 – 100 µm in 10 µm increments, 100 – 600 µm in 50 µm increments

Summary

Preface

Contact Addresses

Provides contact information for Anatomical Pathology divisions.

Welcome

A welcoming message to the user from Thermo Fisher Scientific.

Chapter 1 Important Information and Safety

Symbols and Conventions

Explains symbols and conventions used throughout the manual and on the instrument.

Personal Safety

Details important safety information for personal protection during operation.

General Safety Precautions

Outlines general safety precautions to prevent injury and equipment damage.

Intended Use of the Microtome

Defines the proper use of the microtome in biology, medicine, and industry.

Chapter 2 Introduction

Microtome Description

Provides a detailed overview of the Microm HM355S rotary microtome.

Scope of Delivery

Lists the standard equipment and accessories included with the microtome.

Optional Equipment

Lists optional accessories available for the microtome with their catalog numbers.

Technical Data Sheet

Presents detailed technical specifications and parameters for the microtome.

Chapter 3 Operating Instructions

Setting Up the Microtome

Provides step-by-step instructions for unpacking and setting up the microtome.

Initial Start-Up Procedures

Guides users through the initial setup procedures after unpacking the instrument.

Using the Optional Foot Pedal

Explains how to connect and use the optional foot pedal for operation.

Operating Panel Overview

Details the layout and functions of the microtome's operating panel and its controls.

Display and Key Functions

Explains the functions of various keys and the information displayed on the panel.

Setting Cutting Window

Describes how to set the upper and lower limits of the cutting window.

Starting and Stopping the Cutting Drive

Details the procedures for starting and stopping the motorized cutting drive.

Hand Wheel Brakes

Explains the operation of both mechanical and electronic hand wheel brakes for safety.

Emergency Stop Functionality

Describes the function and use of the emergency stop button and device.

Cutting Process Indicators

Explains how to view section count, sum of thicknesses, and remaining travel.

Section Counter Functionality

Details the function of the section counter and how to reset it.

Section Thickness Sum Display

Describes the display of the sum of cut sections in microns.

Remaining Travel Display

Explains how the remaining travel distance for sectioning is displayed.

Setting Section and Trimming Thickness

Guides on setting the desired section and trimming thicknesses using the control knob.

Trimming and First Cuts

Explains how to perform trimming and obtain first cuts using the instrument.

Fine Feed Adjustment

Details how to use the fine feed for precise specimen adjustment.

Cutting Movement and Retraction

Describes the cutting movements and the retraction function for specimen protection.

Motorized Cutting Drive Operation

Explains how to use the motorized cutting drive and set cutting speed.

Specimen Coarse Feed Control

Details how to use the motorized coarse feed for specimen positioning.

Operating Modes Overview

Introduces the different operating modes available for sectioning.

Selecting Operating Modes

Explains the two methods for selecting operating modes.

Mode Selection via MODE-Button

Describes how to select modes using the dedicated MODE button.

Mode Selection via Menu Knob

Explains how to select modes via the menu using knob [A].

Interval Stroke Mode

Details the interval stroke operating mode.

Single Stroke Mode

Details the single stroke operating mode.

Multi Stroke Mode

Details the multi stroke operating mode.

Continuous Stroke Mode

Details the continuous stroke operating mode.

Setting Time and Date

Instructions for setting the instrument's time and date.

Display Mode Selection

Describes how to select between NORMAL and LARGE display modes.

Disabling the Retraction Function

Explains how to enable or disable the retraction function.

Language Selection for Display

Guides on how to change the display language of the instrument.

Setting Cutting Speed

Details how to adjust and set the cutting speed of the microtome.

Factory Default Settings

Explains how to reset all settings to their factory default values.

Memory Function for First Cuts

Describes the memory function for storing and recalling the "first-cut-position".

Rocking Mode Functionality

Explains how to perform manual sectioning in rocking mode.

Push-In Hand Wheel Handle Operation

Details how to lock and unlock the push-in hand wheel handle for safety.

Adapters for Specimen Clamping

Discusses the different adapters available for specimen clamping.

Non-orienting Adapter

Describes the non-orienting adapter for specimen clamps.

Orienting Adapter for Specimen Orientation

Explains the orienting adapter for aligning specimens with the knife.

Changing and Fastening Specimen Clamps

Provides instructions on how to change and fasten specimen clamps.

Re-adjusting Specimen Clamps

Explains how to readjust specimen clamps for optimal clamping strength.

Specimen Clamping Methods

General overview of specimen clamping methods.

Universal Cassette Clamp

Details the use of the universal cassette clamp.

Adjustable Universal Cassette Clamp

Explains the adjustable universal cassette clamp and its features.

Standard Specimen Clamp

Describes the standard specimen clamp for rectangular and square blocks.

Inserts for Round Specimens and V-Piece

Explains inserts for round specimens and V-inserts for the standard clamp.

Foil Clamp Usage

Details the use of the foil clamp for thin specimens.

Segment Arc and Universal Specimen Holder

Describes holders suitable for clamping small specimens.

Knife and Blade Carriers

Introduces the different types of knife and blade carriers.

Disposable Blade Carrier "ER"

Details the Disposable Blade Carrier "ER" for high and low profile blades.

Using High Profile Blades

Instructions for using high profile blades with the ER carrier.

Clearance Angle Adjustment

Explains how to adjust the clearance angle for optimal sectioning.

Disposable Blade Carrier "E"

Details the Disposable Blade Carrier "E" for various blades.

Moving Blade Carrier on Console

Instructions for moving the blade carrier on the console.

Protection Against Injury

Describes knife guards for protection against injury.

Knife Carrier "C"

Details the Knife Carrier "C" and its functions.

Moving Knife Carrier on Console

Instructions for moving the knife carrier on the console.

Protection Against Injury

Explains knife guards for protection during adjustments.

Central Clamping Plate Types

Describes the clamping plates for C- and D-knives.

Re-adjusting Knife or Blade Carriers

Explains how to readjust knife or blade carriers for proper clamping.

Section Waste Tray Features

Describes the section waste tray and its features.

Large Field Magnifier

Introduces the large field magnifier and its magnification.

Installing the Large Field Magnifier

Provides instructions for mounting the large field magnifier.

Chapter 4 Working with the Microtome

Sectioning Instructions

Provides essential points for cutting usable sections.

Knife/Blade Edge Conditions

Emphasizes the importance of a sharp and clean knife/blade.

Setting the Cutting Angle

Explains how to set the correct knife angle relative to the specimen block.

How to Avoid Sectioning Errors

Offers guidance on preventing common errors during sectioning.

Troubleshooting Common Errors

Lists common problems, their causes, and solutions for troubleshooting.

Chapter 5 Maintenance and Care

Daily Cleaning and Care

Provides daily cleaning and care procedures for the microtome.

General Maintenance

General maintenance recommendations.

Annual Routine Maintenance

Recommends annual routine maintenance by a trained technician.

Service Contract Information

Information about available service contracts for the instrument.

Replacement Work

Details procedures for replacing fuses.

Replacing the Fuses

Step-by-step guide to replacing the instrument's fuses.

Chapter 6 Conditions for Transportation

Returning Instrument for Service

Instructions for returning the instrument for repair or routine maintenance.

Measures for Taking Instrument Out of Operation

Steps to take when preparing the instrument for transport.

Transporting Outside Closed Buildings

Specific measures for transporting the instrument outside buildings.

Disposal After Final Shutdown

Guidance on proper disposal of the instrument at the end of its life.

Chapter 7 Warranty and Certification

Warranty Statement

Outlines warranty provisions and conditions that may invalidate the warranty.

Product Certification

Details the CE marking and compliance with relevant standards.

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