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Thermo Scientific NCS-3500RS User Manual

Thermo Scientific NCS-3500RS
270 pages
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Thermo Scientific Dionex
UltiMate 3000 Series
Nano/Cap System NCS-3500RS
Nano/Cap Pump NCP-3200RS
Operating Instructions
(Original Operating Instructions)
Revision: 2.0
Date: January 2016
© 2016 Thermo Fisher Scientific Inc.
Doc No. 4820.4101

Table of Contents

Questions and Answers:

Thermo Scientific NCS-3500RS Specifications

General IconGeneral
BrandThermo Scientific
ModelNCS-3500RS
CategoryLaboratory Equipment
LanguageEnglish

Summary

1 Introduction

1.2 Safety Information

Crucial safety guidelines covering symbols, precautions, protective equipment, hazardous substances, and environmental hazards.

2 Overview

2.2 Module Configurations

3 Installation

3.4 Connecting the Module

3.5 Setting Up the Module in Chromeleon

3.5.2 Installing the Module

Guides through the process of installing and configuring the module within the Chromeleon Server Configuration program.

3.5.3 Configuring the Module

Explains initial configuration via the General page: Device Type, Virtual Mode, and Module Address.

4 Preparation for Operation (Startup)

7. Pump Module

Details critical pump module setup: seal wash system, flow meter settings, and purging pumps for optimal performance.

8. Column Compartment

Describes setup procedures for the column compartment: valve installation, column installation, and temperature control for chromatography.

4.3 Solvent Reservoirs

4.5 Pump Module

4.5.2 Flow Meter Settings

Explains setting solvent types (ProFlow) or viscosities (Classic) for the NC pump's flow meter for accurate gradient and flow control.

4.5.3 Purging the Pumps

4.5.3.1 Purging the NC Pump

Details purging NC pump heads using a syringe or Chromeleon commands to remove air and old solvents.

4.5.3.1.2 Purging the Flow Meter

Describes purging the NC pump's flow meter to remove air bubbles and solvents, ensuring accurate flow readings.

4.5.3.2 Purging the Loading Pump

Provides steps for purging the loading pump from Chromeleon or the pump display to remove air and solvents.

4.6 Column Compartment

4.7 Equilibrating the System

5 Operation and Maintenance

5.3 Operation from Chromeleon

5.3.1 Connecting to Chromeleon

Guides verifying and connecting the module to Chromeleon client, checking status LEDs and input disablement.

5.3.3 Automated Control

5.4.2.2 Pump Menus

5.4.2.3 Column Compartment Menus

5.5 Information for Operating the Pump

5.5.1 Choosing the Solvents

Offers precautions for solvent selection: miscibility, purity, pH range, and chemical compatibility for system longevity.

5.5.2 Interdependency of Column Pressure and Maximum Flow Rate

Explains column pressure vs. flow rate relationship for NC pump with ProFlow, crucial for performance tuning.

5.5.3 Adding Solvents to the Flow Meter Configuration

Details ProFlow solvent calibration in Chromeleon for defining new solvents and ensuring accurate flow meter performance.

5.5.3.2 Performing a Solvent Viscosity Measurement (Classic Flow Meter)

Explains measuring and saving solvent viscosity for Classic flow meters in Chromeleon via Commands dialog.

5.5.4 Setting the Flow Rate, Flow Acceleration, and Flow Deceleration

5.5.5 Setting the Pressure Limits

5.5.7 Rear Seal Wash System

5.5.7.1 Working with Rear Seal Washing

Explains operating the rear seal wash system: enabling, starting, and stopping wash cycles for pump seal longevity.

5.5.8 Purging the Pumps

5.5.9 Detecting Liquid Leaks in the Pump Module

5.6 Information for Operating the Column Compartment

5.6.1 Turning On Temperature Control

Guides turning column temperature control on/off and setting target temperature in Chromeleon or on the display.

5.7 Special Functions in Chromeleon

5.7.2 Diagnostics

Explains using Chromeleon diagnostics to check module component performance and run tests for troubleshooting.

5.8 Recommendations for Optimally Running Systems (Best Practice)

5.10 Maintenance, and Maintenance and Calibration Intervals

6 Troubleshooting

6.3 Messages on the Pump Display

Lists pump display messages and Chromeleon messages with corresponding remedial actions for troubleshooting.

6.4 Chromeleon Diagnostics Messages

Provides causes and actions for Chromeleon diagnostic test failures (Leak Test, Mixer Frit Test) for troubleshooting.

6.5 Operating Problems

Lists common operating problems, probable causes, and remedial actions for the module and UltiMate 3000 system.

7 Service

7.2 Eliminating Leakage in the Pump Module

Details finding and eliminating pump module leaks, drying the leak sensor, and testing for leakage after repairs.

7.5 Replacing the Check Valve Cartridges

7.6 Pistons and Piston Seals

7.6.1 Visually Inspecting the Pump for Piston Seal Leakage

Details visually inspecting the pump for liquid leaks from piston seals using the seal wash system observation.

7.6.2 Pump Head and Pistons

Provides step-by-step instructions for removing the pump head and pistons from the NC and loading pumps.

7.6.2.2 Installing the Pistons and Pump Head

Guides installing pistons and pump head, including alignment, torque specifications, and proper reassembly.

7.6.3 Replacing the Piston Seals

Explains the importance of piston seals and the procedure for replacing main piston seals in the pump head.

7.6.3.1 Disassembling the Pump Head and Removing the Piston Seals

Details pump head disassembly and piston seal removal, emphasizing cleanliness and component orientation.

7.6.3.3 Installing the Piston Seals and Reassembling the Pump Head

Provides instructions for installing new piston seals and reassembling the pump head, including orientation and cleaning.

7.6.3.4 Replacing the Piston Seals in the Plate of the Seal Wash System

Details replacing piston seals in the plate of the rear seal wash system for continued pump function.

7.6.3.5 Recommended Actions after Main Piston Seal Replacement

Provides recommended actions post-seal replacement: purging, diagnostic tests, and run-in procedures.

7.7 Flow Meter

7.7.1 Replacing the Flow Meter

Details replacing the ProFlow or Classic flow meter, including part numbers, removal, and installation steps.

7.9 Inline Filter (Loading Pump)

7.10 Replacing the Purge Screw (Loading Pump)

7.11 Testing the Loading Pump for Leakage

7.12 Column Switching Valve

7.12.1 Replacing the Column Switching Valve

Details replacing column switching valves, including part numbers, service information updates, and rotor seal replacement.

7.12.2 Replacing the Valve Stator and Rotor Seal

Provides instructions for replacing the valve stator and rotor seal in column switching valves, ensuring proper sealing.

7.14 Updating the Firmware

8 Module-Specific Information

8.2 Loading Pump (Micro Pump)

8.3 Column Compartment

9 ProFlow Flow Meter Upgrade

9.1 Modifying the Module with a ProFlow Flow Meter

Details steps and parts needed to modify NCS/NCP modules with a ProFlow flow meter for nano LC applications.

Follow these steps

Provides the installation steps for the ProFlow flow meter, including software and hardware integration for optimal performance.

9.2 Guidelines on Operating the ProFlow Flow Meter

Offers guidelines for ProFlow flow meter operation: solvent types, purging, diagnostics, and calibration procedures.

10 Technical Information

12 Reference Information

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