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Thermo Scientific HM525 NX UV User Manual

Thermo Scientific HM525 NX UV
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Thermo Scientific HM525 NX
Product Code 956640, 956641,
956650, 956651
Operator Guide
388159 Issue 10

Table of Contents

Questions and Answers:

Thermo Scientific HM525 NX UV Specifications

General IconGeneral
CategoryLaboratory Equipment
BrandThermo Scientific
ModelHM525 NX UV
TypeCryostat
Power Requirements220-240 V, 50/60 Hz
UV LightYes
DisplayTouchscreen
Section Thickness Range1 to 100 µm

Summary

Safety Information

General Safety Precautions

Provides essential safety precautions for operating the instrument.

Chemical Safety Guidelines

Outlines potential hazards and precautions related to chemicals used with the instrument.

Technical Specifications

Cooling Specifications

Provides details on cryobar and chamber cooling capabilities and defrosting options.

Microtome and Sectioning Specifications

Lists specifications for section thickness, retraction, and specimen movement.

Chapter 2 - Instrument Setup

Unpacking and Repacking Safety

Crucial safety guidelines for handling and unpacking the heavy instrument.

Unpacking the Instrument

Step-by-step guide on how to safely remove the instrument from its packaging.

Knife Holder Operations

Solid Knife Holder Setup

Covers inserting, height adjustment, and clearance angle for the solid knife holder.

Disposable Blade Holder EC Setup

Guides on inserting blades and setting angles for the EC holder.

Disposable Blade Holder EC 70 Setup

Instructions for blade insertion and angle adjustment for the EC 70 holder.

Instrument Setup Procedures

Specimen Clamping and Orientation

Guides on clamping and orienting the specimen in the specimen head.

Chapter 3 – Operation

Switching On the Instrument

Provides instructions for powering on the HM525 NX instrument safely.

Setting Preferences

Quick Freezing of Specimens

Explains how to use the cryobar for rapid specimen freezing.

Microtome / Chamber Temperature Control

Setting the Temperature

Details how to adjust the microtome and chamber temperatures.

Section Thickness Adjustment

Setting Trim Section Thickness

Instructions for setting the thickness for trimming passes.

Setting Fine Section Thickness

Guides on setting the desired thickness for fine sectioning.

Instrument Configuration and Settings

Date and Time Configuration

Guides on setting the instrument's date, time, and format.

UV Disinfection Option

Defrost Procedures

Immediate Defrost Execution

Guides on starting an immediate defrost cycle and aborting it.

Scheduled Daily Defrost

Details how to schedule the daily defrost cycle.

System Configuration and Service

Factory Default Settings Reset

Guides on restoring the instrument to its default factory configuration.

Sectioning Procedures

Sectioning and Removal Process

Details the process of cutting sections and removing them from the blade.

Mechanical Handwheel Lock

How to Avoid Malfunctions

Chapter 4 - Troubleshooting

Troubleshooting Guide

Lists common problems, their causes, and recommended solutions.

Tips for Successful Sectioning

Temperature List for Cryo-Sectioning

Chapter 5 – Maintenance and Care

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