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Thermo Scientific HM340E User Manual

Thermo Scientific HM340E
61 pages
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Thermo Scientific
HM340E
Rotary Microtome
Product Code 905190
Operation Manual English
ex Ser. No 44481
387863 Issue 8

Table of Contents

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Thermo Scientific HM340E Specifications

General IconGeneral
TypeMicrotome
Trimming Thickness Setting1 µm increments
Vertical Specimen Stroke60 mm
Specimen RetractionAutomatic
Power Supply100-240 VAC, 50/60 Hz
Operating Temperature Range10°C to 35°C
Operating Humidity Range20% to 80% non-condensing

Summary

Company Information

Symbols

Chapter 1 - Safety Information

General Safety

General safety guidelines for operating the microtome, including warnings and precautions.

Chemical Safety

Safety precautions and guidelines for handling chemicals used with the microtome.

Environment

Information regarding waste disposal and compliance with environmental directives.

Warranty Statement

Details on the product warranty and conditions for its validity.

EMC Statement

Statement on the instrument's compliance with electromagnetic compatibility standards.

Chapter 2 - Introduction

Intended Use

Specifies the device's purpose and applications in diagnostic procedures.

Description of Rotary Microtome HM 340E

Provides an overview of the microtome's features, design, and capabilities.

Scope of Delivery Standard Equipment

Lists all accessories included with the microtome as standard.

Additional Equipment

Details optional accessories available for purchase to enhance microtome functionality.

Technical Data Sheet

Presents detailed technical specifications, performance parameters, and operating conditions.

Chapter 3 – Operating Instructions

Setting up the Microtome

Step-by-step guide for unpacking, positioning, and initial setup of the microtome.

Initial Start-up

Procedure for connecting the operating panel and powering on the instrument for the first time.

Operating Panel

Overview of the microtome's control panel, its layout, and key functions.

Display and Key Function

Explanation of the display elements and the function of various keys on the operating panel.

Cutting Process Indication

Information displayed during the cutting process, including section count and thickness.

Setting Section Thickness and Trimming Thickness

Instructions on how to set precise section and trimming thicknesses for sectioning.

Cutting Movement and Retraction

Details on the microtome's cutting mechanism and the specimen retraction function.

Specimen Course Feed

How to use the motorized coarse feed system for specimen positioning and movement.

Rocking Mode Function

Enables manual sectioning in rocking mode; instructions to start and stop the function.

Adapters for Specimen Clamping

Overview of different adapters for securing specimen clamps to the microtome.

Adapter, Non-Orienting

Adapter for direct, non-orienting fastening of specimen clamps.

Adapter, Orienting, Specimen Orientation

Adapter allowing specimen alignment with the knife/blade.

Specimen Clamping

Methods and accessories for securely clamping specimens for sectioning.

Universal Cassette Clamp

Description of the universal clamp for quick cassette changes.

Universal Cassette Clamp, Adjustable

Details on the adjustable clamp for various cassette sizes.

Standard Specimen Clamp

Clamp designed for rectangular and square paraffin and plastic blocks.

Insert for Round Specimens, V-Insert and V-Distance Piece

Accessories for clamping round specimens or providing V-shaped support.

Knife and Blade Carriers

Information on different carriers for holding knives and blades.

Disposable Blade Carrier ER

Details on the ER model for disposable blades, including blade insertion.

Clearance angle adjustment

Procedure for adjusting the blade's clearance angle for optimal cutting.

Disposable Blade Carrier E

Details on the E model for disposable blades, including blade insertion.

Knife Carrier C

Specific information and instructions for Knife Carrier C, including knife insertion.

Section Waste Tray with Integrated Arm Rest

Description of the waste tray and its integrated arm rest feature.

Large Field Magnifier

Information about the large field magnifier accessory, including its specifications.

Installing the Large Field Magnifier on the Microtone

Instructions for mounting the large field magnifier onto the microtome.

Chapter 4 – Working with the Microtome

Sectioning Instructions

Key points and guidelines for performing effective sectioning with the microtome.

Conditions of Knife/BladeEdge

Requirements for knife/blade sharpness, cleanliness, and condition for optimal sectioning.

Setting the Cutting angle

Procedure for adjusting the knife's cutting angle to achieve desired section quality.

How to Avoid Errors

Tips and procedures to prevent common errors during microtome operation.

Possible Sources of Errors – Cause and Removal

Identifies common problems, their causes, and recommended solutions.

Chapter 5 – Maintenance and Care

Cleaning and Care

Daily cleaning procedures and recommendations for maintaining the microtome.

Maintenance

General maintenance practices and recommendations for instrument upkeep.

Annual Routine Maintenance

Recommendation for yearly maintenance by a trained service technician.

Service Contract

Information on service contracts for ensuring optimal instrument condition.

Replacement Work

Procedures for replacing components, specifically focusing on fuses.

Replacing the Fuses

Step-by-step guide for safely replacing the instrument's power fuses.

Chapter 6 – Conditions for Transportation

Returning the Instrument for Repair or Routine Maintenance

Procedures and precautions for returning the instrument for service or maintenance.

Measure for taking out of Operation

Steps to safely take the microtome out of operation, including temporary storage.

Disposal of the Instrument after Final Shutdown

Guidelines for environmentally responsible disposal of the instrument at end-of-life.

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