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Zeiss Lattice Lightsheet 7 User Manual

Zeiss Lattice Lightsheet 7
80 pages
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Lattice
Lightsheet
7
Safety Information, Setup Requirements and
Instructions
April 2021

Table of Contents

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Zeiss Lattice Lightsheet 7 Specifications

General IconGeneral
Microscope TypeLight sheet microscope
Imaging TechniqueLight Sheet Fluorescence Microscopy (LSFM)
Light SourceLaser-based light source
Excitation Wavelengths405 nm, 488 nm, 561 nm, 640 nm
DetectionsCMOS camera
Detection ChannelsMultiple channels supported
Sample CompatibilityCells, tissues, and small organisms
Sample HandlingAdaptive sample mounting options
Sample ChamberDesigned for live imaging with environmental control
Imaging Modality3D imaging
SoftwareZEN (Zeiss Efficient Navigation) software
IncubationTemperature and CO2 control

Summary

Safety Information

General Safety and System Overview

General safety precautions and system overview.

Explanation of Symbols

Explanation of safety and warning symbols used in the manual.

Device Usage Information

Information on EMC requirements and potential operational disturbances.

Regulatory Compliance

Overview of compliance with relevant industry regulations and guidelines.

Power Requirements

Details on connecting system components to the AC mains grid.

Environmental Requirements

Specifications for operating and storage environment, humidity, and heat emission.

System Setup Instructions

Instructions for safe and proper installation and setup of the system.

Warning and Information Labels

Details and location of warning and information labels affixed to the instrument.

Laser Components and Illumination Handling

Safety precautions for handling laser modules and illumination systems.

Laser Lifetimes

Expected lifetime data for the system's laser modules.

Computer, Data Media, and Data Transfer

Guidelines for safe operation of the computer and data management.

Care, Maintenance, and Service

Information on proper care, maintenance, and service procedures.

Manufacturer Service Maintenance

Recommendations for service agreements and authorized maintenance.

Fuse Replacement and Electrical Connections

Procedure for replacing fuses and managing electrical connections.

Customer Maintenance Tasks

Customer-level maintenance tasks for painted and glass surfaces.

Liquid Cooling System Maintenance

Procedure for maintaining the camera's liquid cooling system.

ZEISS Service Offerings

Overview of ZEISS service products and support options.

User Interface - Component Mounting/Dismounting

Instructions for mounting and dismounting system components.

Meniscus Lens Replacement

Step-by-step guide for replacing the meniscus lens.

Immersion Supply System Procedures

Removing immersion container and priming the system.

Incubator Lid Handling

Instructions for attaching and detaching the incubator lid.

Sample Mounting Frame Exchange

Procedure for removing and inserting sample mounting frames.

Liquid Cooling System Refilling

Steps to refill the liquid cooling system.

Incubation System Operations

Details on heating, gas incubation, and control systems.

Stage Control Operations

Overview of stage control, motion controller, and Ergo pointer.

Detection Module (pco.edge)

Information about the pco.edge sCMOS camera and its connections.

Setup Requirements

Space Requirements

Layout and dimensions for system setup and PC table.

Power Requirements

Details on connecting system components to the AC mains grid.

Physical Dimensions of Items

Table of physical dimensions and weights for system components.

Item Package Dimensions

Dimensions and weights of item packages and shipment crates.

Shipment Crates Contents

Details about the contents of each shipment crate.

Environmental Requirements

Specifications for operating and storage environment, humidity, and heat emission.

Vibration Specifications

Recommended vibration class for system operation.

Laser Specifications

Information on laser properties and attenuation.

Lattice Lightsheet 7 Components

Diagram identifying the main components of the LLS 7 system.

System Overview Diagram

Schematic overview of the entire Lattice Lightsheet 7 system.

Operating Instructions

System Power On/Off

Procedure for powering the system on and off.

ZEN Software Start/Stop

Steps to launch and close the ZEN software application.

System Presetting Procedures

Initial system setup steps before starting operation.

Switching to Operation Mode

Selecting the operation mode after system initialization.

Sample Carrier Selection

Procedure for selecting the correct sample carrier format.

Stage Calibration

Optional step to calibrate the stage for absolute positioning.

Immersion Priming Methods

Methods for priming the immersion system.

Environmental Control Settings

Setting temperature and atmosphere parameters for incubation.

System Operation Procedures

General operation procedures for the system.

Software Control Tabs

Overview of the main control tabs in the software.

Sample Positioning and Finding

Steps for positioning and finding samples using software controls.

Lightsheet Alignment and Stage Levelling

Procedure for aligning the lightsheet and levelling the stage.

Lightsheet Focusing

Steps for focusing the lightsheet for optimal imaging.

Sample Imaging Setup

Procedure for setting up and starting sample imaging scans.

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