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Microscope Type | Light sheet microscope |
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Imaging Technique | Light Sheet Fluorescence Microscopy (LSFM) |
Light Source | Laser-based light source |
Excitation Wavelengths | 405 nm, 488 nm, 561 nm, 640 nm |
Detection | sCMOS camera |
Detection Channels | Multiple channels supported |
Sample Compatibility | Cells, tissues, and small organisms |
Sample Handling | Adaptive sample mounting options |
Sample Chamber | Designed for live imaging with environmental control |
Imaging Modality | 3D imaging |
Software | ZEN (Zeiss Efficient Navigation) software |
Incubation | Temperature and CO2 control |
General safety precautions and system overview.
Explanation of safety and warning symbols used in the manual.
Information on EMC requirements and potential operational disturbances.
Overview of compliance with relevant industry regulations and guidelines.
Details on connecting system components to the AC mains grid.
Specifications for operating and storage environment, humidity, and heat emission.
Instructions for safe and proper installation and setup of the system.
Details and location of warning and information labels affixed to the instrument.
Safety precautions for handling laser modules and illumination systems.
Expected lifetime data for the system's laser modules.
Guidelines for safe operation of the computer and data management.
Information on proper care, maintenance, and service procedures.
Recommendations for service agreements and authorized maintenance.
Procedure for replacing fuses and managing electrical connections.
Customer-level maintenance tasks for painted and glass surfaces.
Procedure for maintaining the camera's liquid cooling system.
Overview of ZEISS service products and support options.
Instructions for mounting and dismounting system components.
Step-by-step guide for replacing the meniscus lens.
Removing immersion container and priming the system.
Instructions for attaching and detaching the incubator lid.
Procedure for removing and inserting sample mounting frames.
Steps to refill the liquid cooling system.
Details on heating, gas incubation, and control systems.
Overview of stage control, motion controller, and Ergo pointer.
Information about the pco.edge sCMOS camera and its connections.
Layout and dimensions for system setup and PC table.
Details on connecting system components to the AC mains grid.
Table of physical dimensions and weights for system components.
Dimensions and weights of item packages and shipment crates.
Details about the contents of each shipment crate.
Specifications for operating and storage environment, humidity, and heat emission.
Recommended vibration class for system operation.
Information on laser properties and attenuation.
Diagram identifying the main components of the LLS 7 system.
Schematic overview of the entire Lattice Lightsheet 7 system.
Procedure for powering the system on and off.
Steps to launch and close the ZEN software application.
Initial system setup steps before starting operation.
Selecting the operation mode after system initialization.
Procedure for selecting the correct sample carrier format.
Optional step to calibrate the stage for absolute positioning.
Methods for priming the immersion system.
Setting temperature and atmosphere parameters for incubation.
General operation procedures for the system.
Overview of the main control tabs in the software.
Steps for positioning and finding samples using software controls.
Procedure for aligning the lightsheet and levelling the stage.
Steps for focusing the lightsheet for optimal imaging.
Procedure for setting up and starting sample imaging scans.