Zeiss LSM710 User Manual (English)

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Zeiss LSM710 User Guide pg. 1

Zeiss LSM710 Confocal User Guide 2023-05-19 edition

- Users must be trained and approved. Work with others until you have mastered the system. Ask JM for help.

- Use iLab for reservations, maximum of 4-hours at a time.

- Do not use gloves to touch confocal switches or the computer.

- Please ask Dr. Murray immediately you notice any issues with the instrument or if you have forgotten any procedures

(jwm2175@cumc.columbia.edu or slack workspace).

Here’s a good but long video demonstration of a LSM710 microscope:

https://www.youtube.com/watch?v=_sCnAPIJGlQ

Zeiss confocal knowledge base

https://www.zeiss.com/microscopy/us/local/zen-knowledge-base-home/zen-knowledge-base-lsm-800-900-980.html

AVOID THESE DAMAGING EVENTS:

• Oil dripping down objective lenses. Use small amount. Gently wipe off oil from lens when there is too

much, also wipe sides of lens. Use only lens paper.

• Bumping Objective Lenses with the stage Insert or your sample (glass slide) always handle the

microscope and slides gently.

• Getting nail polish or other damaging substances on the objectives microscope components (e.g. Oil on

Dry lenses).

• Using very bright laser intensities (>20% will usually cause strong photobleaching).

• Frequently turning on and off the lasers.

• Turning off the power before fan has cooled the argon laser.

How to Start Up the LSM710

Remove dust cover from microscope, inspect the area. See me (8-801F black bldg.) or email

jwm2175@ if you find problems.

Make sure “LASOS panel” little silver switch (6) is on standby (down)

Turn on: (see images below)

(1) Main switch, (2) System/PC, (3), Components

(4) Computer (if not already on).

(5) LASOS argon key from off (0) to on (-)

(6) LASOS panel silver switch to “run”

(7) X-cite mercury lamp, green switch

- Login to computer with your UNI

- Double click ZEN BLACK (not blue!) software, and click “Start”.

(Zen Blue is for analysis only)

It takes ~10min for the green LASOS light to turn on, indicating stabilization of the laser. Make sure

the red light is not on, indicating laser power is too high. If red, turn counterclockwise to green.

Save your images to the D: Drive (this is temporary storage and will be deleted)

Transfer your data to your own external hard drive*No data is allowed on the C: drive and will be

deleted. You can temporarily store images in your folder such as, D: \ USER\ “your folder”. All data

may be deleted after 2 weeks. *Use multiple backups or cloud-based data storage.

Need help?

Do you have a question about the Zeiss LSM710 and is the answer not in the manual?

Zeiss LSM710 Specifications

General

Laser Wavelengths	405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
Resolution (pixels)	Up to 4096 x 4096 pixels
Microscope Type	Confocal Microscope
Scanning Speed	Up to 8 frames per second at 512 x 512 pixels
Scanning Method	Galvanometric Mirrors
Software	ZEN software
Objective Lenses	Various objectives available (5x to 100x, air, water, oil immersion)