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General| Type | Upright Microscope |
|---|---|
| Objective turret | 5 or 6 positions |
| Stage | Mechanical stage |
| Focus | Coarse and fine focusing |
| Objectives | Plan-Neofluar |
| Observation Methods | Brightfield, Darkfield, Phase contrast, Fluorescence |
| Eyepieces | 10x, 12.5x |
| Filter Holder | fluorescence filters |
| Illumination | Halogen lamp (12V/100W) |
Initial steps for Brightfield/DIC microscopy, including specimen placement and light settings.
Procedure for activating the halogen lamp for transmitted light microscopy.
Procedure for activating the mercury lamp for fluorescence microscopy.
Aligning the light path by focusing the specimen and adjusting the analyzer/polarizer.
Using the field diaphragm and centering screws to adjust the light spot.
Adjusting condenser height and aperture diaphragm for optimal image sharpness.
Guidelines for operating the mercury lamp and handling the analyzer to prevent damage.
Techniques to control illumination and minimize sample photobleaching during fluorescence.
Selection and use of filter sets for specific fluorophores.
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