Use of the Axioskop (Zeiss)
The Axioskop is equipped for viewing samples with transmitted light (from a halogen lamp
source) and has optics for: Bright field, Phase Contrast and Differential Interference Contrast
(DIC) microscopy. Samples can also be viewed for fluorescence microscopy using the incident-
light system (front a mercury lamp source).
1) For transmitted light microscopy work, press the
green on/off light switch (1a) at the back-right side of the
microscope while light intensity / level knob is located
next to switch (1b). The halogen lamp is in the vented
box at the bottom rear of the scope (1c).
2) For fluorescence microscopy work, press green on/off light switch (2a) on the front of the
arc lamp power supply box behind the scope (right side- 2b). The mercury lamp is in the vented
housing on the rear of the scope and is above the halogen lamp box (2c).
*Note: the halogen lamp does not need to be on when only viewing fluorescence
Transmitted light microscopy (Brightfield/DIC)
Begin with glass slide/specimen securely in place on stage, light at medium
brightness/temperature (~6).
Ocular's (eyepieces) diopter rings set
at "white dot-to-0-position". In most
cases start with 10 x objective lens in
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