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Zeiss Axioplan 2 User Manual

Zeiss Axioplan 2
92 pages
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Table of Contents

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Zeiss Axioplan 2 Specifications

General IconGeneral
Microscope TypeUpright Microscope
Optical SystemInfinity Color-Corrected System (ICS)
Eyepieces10x
Nosepiece5 or 6 position
StageMechanical stage
IlluminationTransmitted light
Light SourceHalogen 12V 100W
Illumination OptionsBrightfield, Darkfield, Phase contrast, Fluorescence
Camera CompatibilityCompatible with various digital cameras and imaging systems
Objective LensesPlan-Neofluar
Filter SlotsMultiple slots for fluorescence and contrast enhancement filters

Summary

Axioplan 2 and Axiophot 2 Universal Microscopes Operating Manual

Notes on Safe Instrument Operation

Instrument Safety Warnings and Precautions

Covers hazards like crushing, gas discharge lamps, hazardous specimens, hot surfaces, and thermal sensitivity of filters.

Microscope Purpose and Applications

Putting the Microscope into Operation

Unpacking and Initial Microscope Setup

Instructions for unpacking the microscope, emphasizing professional installation for optimal functioning and user attachment of components.

Microscope Setup and Power Management

Connecting and Switching On the Microscope

Details electrical connections, setting up the microscope, and the procedure for switching it on and off.

Axioplan 2 Stand Configurations

Stand Types and Component Versions

Describes different stand types (Manual, E, MOT) and component versions for upper and lower stand parts.

Manual Stand Operation

Manual Stand Controls and Functions

Explains the ON/OFF switch, light intensity, color temperature keys, and other controls for the manual stand operation.

Stand Mechanics: Focusing and Stage

Manual Focusing Drive and Stage Carrier

Explains the manual focusing drive, stage carrier attachment, diffusion disk, and stage mounting procedures.

Stand Adjustments: Stage and Condenser Alignment

Stage Centering and Condenser Setup

Covers stage centering, height adjustment of stage carrier, condenser carrier, and setting the height stop for Köhler illumination.

Motorized Stand (E/MOT) Features

Motorized Stand Specialities and Software Notes

Introduces motorized stand features, electronic system, motor focusing, and the role of microscope software.

Motorized Stand Functions: Light and Focus Control

Incident Light Shutter and Motorized Focusing

Explains incident light shutter, gear switchover, motorized focusing drive, and rapid stage lowering/view functions.

Light Manager and Autofocus Calibration

Light Manager and Parfocality Adjustment

Explains the light manager for automatic illumination adjustment and balancing focusing speed/position for parfocality.

AxioVision Control Software

Software Features and System Requirements

Details AxioVision Control capabilities, including remote control, and lists hardware and software requirements.

AxioVision Control Electrical Connections

Connecting Microscope and Photo Module

Provides instructions for electrical connections between the microscope, PC, and the Axiophot 2 photo module.

System Overview: Microscope Condensers

Microscope Components: Overview

Microscope Components: Illuminator Overview

Microscope Illuminator Specifications

Presents an overview table of microscope illuminators (HAL 100, HBO 50, XBO 75), their applications, component parts, and catalog numbers.

HAL 100 Halogen Illuminator Details

Halogen Lamp Adjustment and Replacement

Adjusting and Changing the HAL Lamp

Provides step-by-step instructions for adjusting the halogen lamp filament and safely changing the lamp.

Microscope Tubes Overview

Microscope Eyepieces Overview

Intermediate Tubes Overview

Reflector Modules Overview

Objectives: General Information and Attachment

Objective Specifications and Mounting

Provides general information on objectives, explains ICS optics, and details the process of screwing objectives into the nosepiece.

Microscope Stages Overview

Mechanical and Rotary Stage Specifications

Lists various mechanical and rotary stages, their specifications, comments on usage, and catalog numbers.

Condensers for Transmitted Light Overview

Transmitted Light Condenser Catalog

Lists and describes various condensers for transmitted light, including their specifications and applications.

Motorized Condenser Attachment

Analyzers, Compensators, and DIC Sliders Overview

Analyzers, Compensators, and DIC Slider Specifications

Lists analyzers, compensators, auxiliary objects, and DIC sliders, detailing their specifications and applications.

Axiophot 2 Photo Module Overview

Axiophot 2 Photo Module Features

Introduces the Axiophot 2 Photo module, its capabilities, and identifies key parts like cameras and film cassettes.

Axiophot 2 Photo Module: Film Cassette Operation

Axiophot 2 Photo Module: Large Format and TV Cameras

Microscopy Techniques: Transmitted Light Brightfield

Setting Up Transmitted Light Brightfield Illumination

Guides the user through setting up transmitted light brightfield illumination using the Köhler principle.

Microscopy Techniques: Transmitted Light Darkfield

Microscopy Techniques: Darkfield Illumination Low Magnification

Microscopy Techniques: Phase Contrast

Microscopy Techniques: DIC in Transmitted Light

Microscopy Techniques: Transmitted-light Polarization

Microscopy Techniques: Incident Light Brightfield

Setting Up Incident Light Brightfield Illumination

Provides instructions for setting up incident light brightfield illumination using the Köhler principle and adjusting components.

Microscopy Techniques: Incident Light Darkfield

Microscopy Techniques: DIC in Incident Light

Microscopy Techniques: Incident-light Polarization

Microscopy Techniques: Epi-fluorescence

Epi-fluorescence Setup and Considerations

Guides the user through setting up epi-fluorescence, including lamp selection, filter combinations, and specimen preparation.

Microscopy Techniques: Fluorescence Photography

Care and Maintenance: General Procedures

General Care, Dust Prevention, and Moving

Covers general instrument care, protection against dust and humidity, and safe procedures for moving the microscope.

Care and Maintenance: Lamp and Fuse Management

Technical Data: Environmental and Safety Specifications

Technical Data: Lamp and Power Specifications

Interface Description: General Functions

Interface Description: Communication Protocols

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