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Zeiss Axio Imager 2 - User Manual

Zeiss Axio Imager 2
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Axio Imager 2
Upright microscope
Operating Manual

Table of Contents

Questions and Answers

Summary

Introduction

Notes on Device Safety

General safety instructions and warning symbols for the microscope.

Warning Labels

Explains warning labels and hazard symbols used in the manual.

Warranty Information

Details the warranty terms and conditions for the instrument.

Other Operating Manuals

Lists related operating manuals for peripheral equipment and software.

Overall View of Axio Imager 2 - Coded

Provides an illustration and list of components for the coded version.

Overall View of Axio Imager 2 - Motorized

Provides an illustration and list of components for the motorized version.

Instrument Description

Name and Designated Use

Describes the manufacturer's designations and intended applications of the microscope.

Instrument Description and Main Features

Details the design principles, ergonomics, and microscopy techniques.

Equipment and Compatibility Table

Lists components and their compatibility across different stand versions.

System Overview of Axio Imager 2

Illustrates system components like condensers, stages, tubes, etc.

First-Time Set-up

Unpacking and Installing the Microscope

Provides instructions for unpacking and initial installation of the microscope.

Installation Conditions and Notes

Outlines ambient conditions and environmental considerations for installation.

Attaching or Changing Binocular Tube or Phototube

Step-by-step guide for attaching or replacing binocular tubes.

Mounting the Tube Lens Turret

Instructions for mounting the tube lens turret onto the stand.

Inserting the Eyepieces and Auxiliary Microscope

Guide for inserting eyepieces and the auxiliary microscope.

Inserting the Eyepiece Reticles

Details on inserting eyepiece reticles and their placement.

Compensation of Ametropia when Eyepiece Reticles are Used

Explains how to compensate for eye differences when using eyepiece reticles.

Inserting the Fold-over Eyecups

Instructions for attaching fold-over eyecups to eyepieces.

Setting the Interpupillary Distance on the Binocular Tube

Explains how to adjust the interpupillary distance for comfortable viewing.

Setting the Viewing Height

Describes how to adjust the viewing height of the binocular tubes.

Connecting Equipment to the Camera Port on the Binocular Phototube

Guide for connecting cameras and other equipment to the phototube.

Screwing in Objectives

Instructions for correctly installing objectives into the nosepiece.

Inserting Reflector Turret, 6 x20 Compensator Mount or 4-Position Modulator Turret

Steps for inserting reflector turrets and related mounts.

Installing and Removing P&C Reflector Modules

Procedures for installing and removing reflector modules.

Changing the Filter Set in the Reflector Module FL P&C

Guide for changing filter sets within the FL P&C reflector module.

Changing the Beam Splitter in the Reflector Module FL P&C

Instructions for changing the beam splitter in the FL P&C reflector module.

Attaching or Changing the Condenser

Steps for attaching or changing the microscope condenser.

Replacing the DIC Prism on the Universal Condenser

Procedure for replacing the DIC prism in the universal condenser.

Changing the DIC Prism of the Modulator Turret (mot.)

Instructions for changing the DIC prism in the motorized modulator turret.

Changing the Stage Carrier

Guide for removing and attaching the stage carrier.

Setting Stops for the Adjustment Range of the Stage Carrier Dovetail Guide

Details on setting limit screws for the stage carrier dovetail guide.

Setting the Dovetail Guide of the Stage Carrier

Instructions for adjusting the dovetail guide for specimen space expansion.

Attaching the Condenser Carrier to the Transmitted-light; Reflected-light Stage Carrier

Steps for attaching the condenser carrier to the stage carrier.

Changing the Fine-Drive Knob or the Fine-Drive Disk on the Stand

Guide for swapping fine-drive knobs or disks on the microscope stand.

Insert the Filter in the Double Filter Wheel

Instructions for inserting filters into the double filter wheel.

Axio Imager.Z2; Z2 m High-performance Focus

Information on activating and deactivating the high-performance focus system.

Activating the High-performance Focus

Step-by-step guide to activate the high-performance focus feature.

Deactivating the High-performance Focus

Procedure for deactivating the high-performance focus system.

Mounting the TFT Display on the Motorized Stand

Instructions for mounting the TFT display onto the motorized stand.

Attaching the TFT Display to the Docking Station

Guide for connecting the TFT display to the docking station.

Installing the Focus Linear Sensor

Procedure for installing the focus linear sensor.

Assembling the Multidiscussion Equipment

Refers to separate manual for multidiscussion equipment assembly.

Connecting to Power

Instructions for connecting the microscope to a power source.

HAL 100 Halogen Illuminator

Information about the HAL 100 halogen illuminator.

Attaching the HAL 100 Halogen Illuminator

Step-by-step guide for attaching the HAL 100 illuminator.

Separate Power Supply of the HAL 100 Halogen Illuminator for Reflected Light

Details on connecting a separate power supply for reflected light operation.

Adjusting the HAL 100 Halogen Illuminator

Guide for coarse and fine adjustment of the HAL 100 illuminator.

Replacing the HAL 100 Halogen Lamp

Instructions for safely replacing the halogen lamp.

Installing the LED Illuminator for Transmitted Light

Guide for installing the LED illuminator for transmitted light.

VIS-LED Attachment Lamp for Transmitted Light

Instructions for installing the VIS-LED attachment lamp.

Adjusting the VIS-LED Attachment Lamp

Guide for adjusting the VIS-LED attachment lamp.

Mounting the microLED Attachment Lamp for Transmitted Light

Instructions for mounting the microLED attachment lamp.

HBO 100 Illuminator

Information about the HBO 100 illuminator.

Inserting the HBO 103 W;2 Mercury Vapor Short-Arc Lamp

Steps for inserting the mercury vapor lamp into the illuminator housing.

Attaching the HBO 100 Illuminator

Guide for attaching the HBO 100 illuminator and its transformer.

Aligning the HBO 100 Illuminator

Procedure for aligning the HBO 100 illuminator.

Setting up the Switching Mirror for Two Illuminators (Manual or Motorized)

Instructions for setting up the switching mirror for dual illuminator use.

Electrical Connections on the Rear of the Microscope

Details electrical connection ports on the microscope rear.

Mount Sample Stage

Guide for mounting different types of sample stages.

Fixed Mechanical Stage 75 x50 R

Instructions for removing and attaching the fixed mechanical stage.

Rotatable Mechanical Stage 75 x50;240° R

Procedures for removing and attaching the rotatable mechanical stage.

Centering the Stage

Guide for centering the stage for optimal viewing.

Adjusting the Stage Drive Length on the Ergonomic Drive

How to adjust the stage drive length for better ergonomics.

Removing and Attaching Additional Sleeves

Instructions for fitting or removing optional drive knob sleeves.

Adjusting the Action (Torque) of the Two Drive Knobs of the Ergonomic Drive

Guide for adjusting the torque of the ergonomic drive knobs.

Changing the Specimen Holder

Instructions for changing the specimen holder on the mechanical stage.

Rotary Stage Pol

Information about the Pol rotary stage.

Removing the Rotary Stage Pol

Procedure for removing the Pol rotary stage.

Attaching Rotary Stage Pol

Steps for attaching the Pol rotary stage.

Centering Rotary Stage Pol

Guide for centering the rotary stage for optimal viewing.

Centering Objectives

Procedure for centering objectives with the rotary stage.

Mechanical Stage 75 x50 mot. CAN

Information on the motorized mechanical stage.

Assembling and Connecting 75 x50 mot. CAN Mechanical Stage with Coaxial Drive

Steps for assembling and connecting the motorized stage with coaxial drive.

Connecting Mechanical Stage 75 x50 mot. CAN (Version 2 and mot. standard)

Details on connecting the motorized stage via CAN bus.

Assembling Mechanical Stage 75 x 50 mot. CAN and Connecting Joystick; Trackball

Guide for assembling the stage and connecting control peripherals.

Mounting the Specimen Holder on the Mechanical Stage

Instructions for mounting the specimen holder.

CAN;USB Converter

Details on connecting the CAN/USB converter for PC communication.

Docking Station

Information on using the docking station with the motorized stand.

Installing Polarizer D, Fixed

Guide for installing the fixed polarizer.

Operation

Coded Axio Imager 2, Coded, Operation and Function Controls

Overview of controls and functions for the coded Axio Imager 2.

Axio Imager 2 Operation and Function Controls (Motorized Version)

Details operations and controls specific to the motorized version.

Switching the Microscope and HAL 100 Illuminator On;Off

Instructions for powering the microscope and HAL 100 illuminator on and off.

Switching the HBO 100 On;Off

Guide for switching the HBO 100 illuminator on and off.

Binocular Phototube 30°;25 mot. with Two Camera Ports (2 TV Tube mot.)

Details on the motorized binocular phototube with two camera ports.

The Light Manager

Explains the Light Manager function for optimizing illumination settings.

The Contrast Manager

Explains the Contrast Manager for automatic contrast method settings.

Operating the Motorized Microscope via the Touchscreen of the TFT Display

Guide to operating the motorized microscope using the TFT touchscreen.

Screen Layout

Describes the main areas of the TFT display screen layout.

Navigation Button Bar

Details the function of navigation buttons on the TFT display.

Status Line

Explains the status line providing information on current settings.

Control Area

Describes the control area where component operations take place.

Menu Structure

Outlines the menu structure of the microscope's software interface.

Home Page

Describes the initial screen displayed after the microscope is started.

Favorite Page

How to set and use a favorite page for quick access.

Microscope Page

Accessing operation pages like Turret, Illumination, Auto. operation, XYZ.

Revolver Page

Details the Revolver page for configuring objectives and other settings.

Light Page

Details functions for light path and intensity control.

Automatic Operation Page

Accessing Soft Keys and HW Settings for automatic operations.

XYZ Page

Managing stage position and measurement functions.

Config Page

Accessing configuration settings for components, extras, and info.

Components Page

Configuring objectives, reflector turrets, and other components.

Extras Page

Accessing Light Manager, Contrast Manager, and other settings.

Info Page

Displays firmware version information.

User Page

Accessing user selection, configuration, and admin pages.

Display Page

Adjusting TFT display brightness and turning it on/off.

Capturing Large Overviews (Tiles or MosaiX Images) on Axio Imager 2

Guide to capturing large overview images using tiling or MosaiX.

Relocate an Event on a Sample Carrier

Procedure for relocating saved events on a sample carrier.

Microscope Equipment and Basic Settings

Basic settings for capturing overview shots.

Calibration of the Stage

Steps for calibrating the microscope stage.

Automatic Component Recognition and User-Defined Component Equipment

Explains the ACR system and custom component configuration.

Programming of Rewritable ACR Reflector Modules

Guide for programming rewritable ACR reflector modules.

Configuration of User-Defined Filter Wheels

Details on configuring user-defined filter sets using software.

Configuration of Motorized Condensers

Information on configuring motorized condensers for reproducible settings.

Illumination and Contrast Methods

Overview of illumination and contrast techniques.

Setting Transmitted-Light Brightfield According to KÖHLER

Step-by-step guide for setting up Köhler illumination for brightfield.

Setting the Transmitted-Light Darkfield

Procedure for setting up transmitted-light darkfield illumination.

Setting Transmitted-Light Phase Contrast

Guide for setting up transmitted-light phase contrast microscopy.

Setting Transmitted-Light Differential Interference Contrast (DIC)

Procedure for setting up transmitted-light DIC contrast.

Setting Transmitted-Light Polarization for Orthoscopic Observation - Determining the Optical Character of Crystals

Setting up transmitted-light polarization for observing crystal optical characteristics.

Measuring Path Differences

How to measure path differences using compensators.

Transmitted-Light Circular Polarization Contrast

Procedure for setting up transmitted-light circular polarization contrast.

Setting Transmitted-Light Polarization for Conoscopic Observation - Determining the Optical Character of Crystals

Setting up transmitted-light polarization for conoscopic observation of crystals.

Setting Reflected-Light Brightfield

Guide for setting up reflected-light brightfield microscopy.

Setting Reflected-Light Darkfield

Procedure for setting up reflected-light darkfield microscopy.

Setting Reflected-Light DIC and Reflected-Light C-DIC

Steps for setting up reflected-light DIC and C-DIC contrast.

Setting Reflected-Light TIC

Procedure for setting up reflected-light TIC technique.

Setting Reflected Light Fluorescence

Guide for setting up reflected-light fluorescence microscopy.

Setting Reflected-Light Polarization - Detection of Bireflection and Reflection Pleochroism

Procedure for detecting bireflection and pleochroism in reflected light.

Care, Maintenance, Troubleshooting and Service

Instrument Care

General guidelines for maintaining and cleaning the microscope.

Instrument Maintenance

Details on regular maintenance and checks for the instrument.

Performing Checks

Routine checks to ensure proper functioning and safety.

Changing Fuses on the Manual Stand

Instructions for replacing fuses on the manual stand.

Changing Fuses on the VP232-2 CAN Power Supply

Procedure for changing fuses on the motorized stand's power supply.

Troubleshooting

Table of common problems, their causes, and solutions.

Spares, Consumables and Tools

List of spare parts, consumables, and tools for the microscope.

Requesting Service

Information on how to contact ZEISS Service for repairs.

Appendix

List of Abbreviations

Glossary of abbreviations used throughout the manual.

Index

Alphabetical index of topics covered in the manual for quick reference.

Industrial Property Rights

Lists patents and registered utility models related to the instrument.

Summary

Introduction

Notes on Device Safety

General safety instructions and warning symbols for the microscope.

Warning Labels

Explains warning labels and hazard symbols used in the manual.

Warranty Information

Details the warranty terms and conditions for the instrument.

Other Operating Manuals

Lists related operating manuals for peripheral equipment and software.

Overall View of Axio Imager 2 - Coded

Provides an illustration and list of components for the coded version.

Overall View of Axio Imager 2 - Motorized

Provides an illustration and list of components for the motorized version.

Instrument Description

Name and Designated Use

Describes the manufacturer's designations and intended applications of the microscope.

Instrument Description and Main Features

Details the design principles, ergonomics, and microscopy techniques.

Equipment and Compatibility Table

Lists components and their compatibility across different stand versions.

System Overview of Axio Imager 2

Illustrates system components like condensers, stages, tubes, etc.

First-Time Set-up

Unpacking and Installing the Microscope

Provides instructions for unpacking and initial installation of the microscope.

Installation Conditions and Notes

Outlines ambient conditions and environmental considerations for installation.

Attaching or Changing Binocular Tube or Phototube

Step-by-step guide for attaching or replacing binocular tubes.

Mounting the Tube Lens Turret

Instructions for mounting the tube lens turret onto the stand.

Inserting the Eyepieces and Auxiliary Microscope

Guide for inserting eyepieces and the auxiliary microscope.

Inserting the Eyepiece Reticles

Details on inserting eyepiece reticles and their placement.

Compensation of Ametropia when Eyepiece Reticles are Used

Explains how to compensate for eye differences when using eyepiece reticles.

Inserting the Fold-over Eyecups

Instructions for attaching fold-over eyecups to eyepieces.

Setting the Interpupillary Distance on the Binocular Tube

Explains how to adjust the interpupillary distance for comfortable viewing.

Setting the Viewing Height

Describes how to adjust the viewing height of the binocular tubes.

Connecting Equipment to the Camera Port on the Binocular Phototube

Guide for connecting cameras and other equipment to the phototube.

Screwing in Objectives

Instructions for correctly installing objectives into the nosepiece.

Inserting Reflector Turret, 6 x20 Compensator Mount or 4-Position Modulator Turret

Steps for inserting reflector turrets and related mounts.

Installing and Removing P&C Reflector Modules

Procedures for installing and removing reflector modules.

Changing the Filter Set in the Reflector Module FL P&C

Guide for changing filter sets within the FL P&C reflector module.

Changing the Beam Splitter in the Reflector Module FL P&C

Instructions for changing the beam splitter in the FL P&C reflector module.

Attaching or Changing the Condenser

Steps for attaching or changing the microscope condenser.

Replacing the DIC Prism on the Universal Condenser

Procedure for replacing the DIC prism in the universal condenser.

Changing the DIC Prism of the Modulator Turret (mot.)

Instructions for changing the DIC prism in the motorized modulator turret.

Changing the Stage Carrier

Guide for removing and attaching the stage carrier.

Setting Stops for the Adjustment Range of the Stage Carrier Dovetail Guide

Details on setting limit screws for the stage carrier dovetail guide.

Setting the Dovetail Guide of the Stage Carrier

Instructions for adjusting the dovetail guide for specimen space expansion.

Attaching the Condenser Carrier to the Transmitted-light; Reflected-light Stage Carrier

Steps for attaching the condenser carrier to the stage carrier.

Changing the Fine-Drive Knob or the Fine-Drive Disk on the Stand

Guide for swapping fine-drive knobs or disks on the microscope stand.

Insert the Filter in the Double Filter Wheel

Instructions for inserting filters into the double filter wheel.

Axio Imager.Z2; Z2 m High-performance Focus

Information on activating and deactivating the high-performance focus system.

Activating the High-performance Focus

Step-by-step guide to activate the high-performance focus feature.

Deactivating the High-performance Focus

Procedure for deactivating the high-performance focus system.

Mounting the TFT Display on the Motorized Stand

Instructions for mounting the TFT display onto the motorized stand.

Attaching the TFT Display to the Docking Station

Guide for connecting the TFT display to the docking station.

Installing the Focus Linear Sensor

Procedure for installing the focus linear sensor.

Assembling the Multidiscussion Equipment

Refers to separate manual for multidiscussion equipment assembly.

Connecting to Power

Instructions for connecting the microscope to a power source.

HAL 100 Halogen Illuminator

Information about the HAL 100 halogen illuminator.

Attaching the HAL 100 Halogen Illuminator

Step-by-step guide for attaching the HAL 100 illuminator.

Separate Power Supply of the HAL 100 Halogen Illuminator for Reflected Light

Details on connecting a separate power supply for reflected light operation.

Adjusting the HAL 100 Halogen Illuminator

Guide for coarse and fine adjustment of the HAL 100 illuminator.

Replacing the HAL 100 Halogen Lamp

Instructions for safely replacing the halogen lamp.

Installing the LED Illuminator for Transmitted Light

Guide for installing the LED illuminator for transmitted light.

VIS-LED Attachment Lamp for Transmitted Light

Instructions for installing the VIS-LED attachment lamp.

Adjusting the VIS-LED Attachment Lamp

Guide for adjusting the VIS-LED attachment lamp.

Mounting the microLED Attachment Lamp for Transmitted Light

Instructions for mounting the microLED attachment lamp.

HBO 100 Illuminator

Information about the HBO 100 illuminator.

Inserting the HBO 103 W;2 Mercury Vapor Short-Arc Lamp

Steps for inserting the mercury vapor lamp into the illuminator housing.

Attaching the HBO 100 Illuminator

Guide for attaching the HBO 100 illuminator and its transformer.

Aligning the HBO 100 Illuminator

Procedure for aligning the HBO 100 illuminator.

Setting up the Switching Mirror for Two Illuminators (Manual or Motorized)

Instructions for setting up the switching mirror for dual illuminator use.

Electrical Connections on the Rear of the Microscope

Details electrical connection ports on the microscope rear.

Mount Sample Stage

Guide for mounting different types of sample stages.

Fixed Mechanical Stage 75 x50 R

Instructions for removing and attaching the fixed mechanical stage.

Rotatable Mechanical Stage 75 x50;240° R

Procedures for removing and attaching the rotatable mechanical stage.

Centering the Stage

Guide for centering the stage for optimal viewing.

Adjusting the Stage Drive Length on the Ergonomic Drive

How to adjust the stage drive length for better ergonomics.

Removing and Attaching Additional Sleeves

Instructions for fitting or removing optional drive knob sleeves.

Adjusting the Action (Torque) of the Two Drive Knobs of the Ergonomic Drive

Guide for adjusting the torque of the ergonomic drive knobs.

Changing the Specimen Holder

Instructions for changing the specimen holder on the mechanical stage.

Rotary Stage Pol

Information about the Pol rotary stage.

Removing the Rotary Stage Pol

Procedure for removing the Pol rotary stage.

Attaching Rotary Stage Pol

Steps for attaching the Pol rotary stage.

Centering Rotary Stage Pol

Guide for centering the rotary stage for optimal viewing.

Centering Objectives

Procedure for centering objectives with the rotary stage.

Mechanical Stage 75 x50 mot. CAN

Information on the motorized mechanical stage.

Assembling and Connecting 75 x50 mot. CAN Mechanical Stage with Coaxial Drive

Steps for assembling and connecting the motorized stage with coaxial drive.

Connecting Mechanical Stage 75 x50 mot. CAN (Version 2 and mot. standard)

Details on connecting the motorized stage via CAN bus.

Assembling Mechanical Stage 75 x 50 mot. CAN and Connecting Joystick; Trackball

Guide for assembling the stage and connecting control peripherals.

Mounting the Specimen Holder on the Mechanical Stage

Instructions for mounting the specimen holder.

CAN;USB Converter

Details on connecting the CAN/USB converter for PC communication.

Docking Station

Information on using the docking station with the motorized stand.

Installing Polarizer D, Fixed

Guide for installing the fixed polarizer.

Operation

Coded Axio Imager 2, Coded, Operation and Function Controls

Overview of controls and functions for the coded Axio Imager 2.

Axio Imager 2 Operation and Function Controls (Motorized Version)

Details operations and controls specific to the motorized version.

Switching the Microscope and HAL 100 Illuminator On;Off

Instructions for powering the microscope and HAL 100 illuminator on and off.

Switching the HBO 100 On;Off

Guide for switching the HBO 100 illuminator on and off.

Binocular Phototube 30°;25 mot. with Two Camera Ports (2 TV Tube mot.)

Details on the motorized binocular phototube with two camera ports.

The Light Manager

Explains the Light Manager function for optimizing illumination settings.

The Contrast Manager

Explains the Contrast Manager for automatic contrast method settings.

Operating the Motorized Microscope via the Touchscreen of the TFT Display

Guide to operating the motorized microscope using the TFT touchscreen.

Screen Layout

Describes the main areas of the TFT display screen layout.

Navigation Button Bar

Details the function of navigation buttons on the TFT display.

Status Line

Explains the status line providing information on current settings.

Control Area

Describes the control area where component operations take place.

Menu Structure

Outlines the menu structure of the microscope's software interface.

Home Page

Describes the initial screen displayed after the microscope is started.

Favorite Page

How to set and use a favorite page for quick access.

Microscope Page

Accessing operation pages like Turret, Illumination, Auto. operation, XYZ.

Revolver Page

Details the Revolver page for configuring objectives and other settings.

Light Page

Details functions for light path and intensity control.

Automatic Operation Page

Accessing Soft Keys and HW Settings for automatic operations.

XYZ Page

Managing stage position and measurement functions.

Config Page

Accessing configuration settings for components, extras, and info.

Components Page

Configuring objectives, reflector turrets, and other components.

Extras Page

Accessing Light Manager, Contrast Manager, and other settings.

Info Page

Displays firmware version information.

User Page

Accessing user selection, configuration, and admin pages.

Display Page

Adjusting TFT display brightness and turning it on/off.

Capturing Large Overviews (Tiles or MosaiX Images) on Axio Imager 2

Guide to capturing large overview images using tiling or MosaiX.

Relocate an Event on a Sample Carrier

Procedure for relocating saved events on a sample carrier.

Microscope Equipment and Basic Settings

Basic settings for capturing overview shots.

Calibration of the Stage

Steps for calibrating the microscope stage.

Automatic Component Recognition and User-Defined Component Equipment

Explains the ACR system and custom component configuration.

Programming of Rewritable ACR Reflector Modules

Guide for programming rewritable ACR reflector modules.

Configuration of User-Defined Filter Wheels

Details on configuring user-defined filter sets using software.

Configuration of Motorized Condensers

Information on configuring motorized condensers for reproducible settings.

Illumination and Contrast Methods

Overview of illumination and contrast techniques.

Setting Transmitted-Light Brightfield According to KÖHLER

Step-by-step guide for setting up Köhler illumination for brightfield.

Setting the Transmitted-Light Darkfield

Procedure for setting up transmitted-light darkfield illumination.

Setting Transmitted-Light Phase Contrast

Guide for setting up transmitted-light phase contrast microscopy.

Setting Transmitted-Light Differential Interference Contrast (DIC)

Procedure for setting up transmitted-light DIC contrast.

Setting Transmitted-Light Polarization for Orthoscopic Observation - Determining the Optical Character of Crystals

Setting up transmitted-light polarization for observing crystal optical characteristics.

Measuring Path Differences

How to measure path differences using compensators.

Transmitted-Light Circular Polarization Contrast

Procedure for setting up transmitted-light circular polarization contrast.

Setting Transmitted-Light Polarization for Conoscopic Observation - Determining the Optical Character of Crystals

Setting up transmitted-light polarization for conoscopic observation of crystals.

Setting Reflected-Light Brightfield

Guide for setting up reflected-light brightfield microscopy.

Setting Reflected-Light Darkfield

Procedure for setting up reflected-light darkfield microscopy.

Setting Reflected-Light DIC and Reflected-Light C-DIC

Steps for setting up reflected-light DIC and C-DIC contrast.

Setting Reflected-Light TIC

Procedure for setting up reflected-light TIC technique.

Setting Reflected Light Fluorescence

Guide for setting up reflected-light fluorescence microscopy.

Setting Reflected-Light Polarization - Detection of Bireflection and Reflection Pleochroism

Procedure for detecting bireflection and pleochroism in reflected light.

Care, Maintenance, Troubleshooting and Service

Instrument Care

General guidelines for maintaining and cleaning the microscope.

Instrument Maintenance

Details on regular maintenance and checks for the instrument.

Performing Checks

Routine checks to ensure proper functioning and safety.

Changing Fuses on the Manual Stand

Instructions for replacing fuses on the manual stand.

Changing Fuses on the VP232-2 CAN Power Supply

Procedure for changing fuses on the motorized stand's power supply.

Troubleshooting

Table of common problems, their causes, and solutions.

Spares, Consumables and Tools

List of spare parts, consumables, and tools for the microscope.

Requesting Service

Information on how to contact ZEISS Service for repairs.

Appendix

List of Abbreviations

Glossary of abbreviations used throughout the manual.

Index

Alphabetical index of topics covered in the manual for quick reference.

Industrial Property Rights

Lists patents and registered utility models related to the instrument.

Zeiss Axio Imager 2 Specifications

General IconGeneral
Microscope TypeUpright Microscope
FocusingCoaxial coarse and fine focusing
Optical SystemInfinity-corrected optical system
IlluminationTransmitted Light, Reflected Light (Epi-fluorescence)
Light SourcesHalogen, LED, or mercury vapor
Objective LensesPlan-Apochromat, Achroplan, EC Plan-Neofluar
StageMotorized or manual stage options
Focus DriveMotorized or manual focus drive
Camera CompatibilityCompatible with various digital cameras
Camera PortsC-mount
SoftwareZEN software
Software CompatibilityWindows
Contrast MethodsBrightfield, Darkfield, Phase Contrast, DIC, Fluorescence
ModularityHighly modular system

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