What causes double images in brightfield with Zeiss Axio Imager 2?
If you're seeing double images in brightfield examinations with your Zeiss Microscope, the DIC slider may be in the light path. Remove the DIC slider from the light path.
General safety instructions and warning symbols for the microscope.
Explains warning labels and hazard symbols used in the manual.
Details the warranty terms and conditions for the instrument.
Lists related operating manuals for peripheral equipment and software.
Provides an illustration and list of components for the coded version.
Provides an illustration and list of components for the motorized version.
Describes the manufacturer's designations and intended applications of the microscope.
Details the design principles, ergonomics, and microscopy techniques.
Lists components and their compatibility across different stand versions.
Illustrates system components like condensers, stages, tubes, etc.
Provides instructions for unpacking and initial installation of the microscope.
Outlines ambient conditions and environmental considerations for installation.
Step-by-step guide for attaching or replacing binocular tubes.
Instructions for mounting the tube lens turret onto the stand.
Guide for inserting eyepieces and the auxiliary microscope.
Details on inserting eyepiece reticles and their placement.
Explains how to compensate for eye differences when using eyepiece reticles.
Instructions for attaching fold-over eyecups to eyepieces.
Explains how to adjust the interpupillary distance for comfortable viewing.
Describes how to adjust the viewing height of the binocular tubes.
Guide for connecting cameras and other equipment to the phototube.
Instructions for correctly installing objectives into the nosepiece.
Steps for inserting reflector turrets and related mounts.
Procedures for installing and removing reflector modules.
Guide for changing filter sets within the FL P&C reflector module.
Instructions for changing the beam splitter in the FL P&C reflector module.
Steps for attaching or changing the microscope condenser.
Procedure for replacing the DIC prism in the universal condenser.
Instructions for changing the DIC prism in the motorized modulator turret.
Guide for removing and attaching the stage carrier.
Details on setting limit screws for the stage carrier dovetail guide.
Instructions for adjusting the dovetail guide for specimen space expansion.
Steps for attaching the condenser carrier to the stage carrier.
Guide for swapping fine-drive knobs or disks on the microscope stand.
Instructions for inserting filters into the double filter wheel.
Information on activating and deactivating the high-performance focus system.
Step-by-step guide to activate the high-performance focus feature.
Procedure for deactivating the high-performance focus system.
Instructions for mounting the TFT display onto the motorized stand.
Guide for connecting the TFT display to the docking station.
Procedure for installing the focus linear sensor.
Refers to separate manual for multidiscussion equipment assembly.
Instructions for connecting the microscope to a power source.
Information about the HAL 100 halogen illuminator.
Step-by-step guide for attaching the HAL 100 illuminator.
Details on connecting a separate power supply for reflected light operation.
Guide for coarse and fine adjustment of the HAL 100 illuminator.
Instructions for safely replacing the halogen lamp.
Guide for installing the LED illuminator for transmitted light.
Instructions for installing the VIS-LED attachment lamp.
Guide for adjusting the VIS-LED attachment lamp.
Instructions for mounting the microLED attachment lamp.
Information about the HBO 100 illuminator.
Steps for inserting the mercury vapor lamp into the illuminator housing.
Guide for attaching the HBO 100 illuminator and its transformer.
Procedure for aligning the HBO 100 illuminator.
Instructions for setting up the switching mirror for dual illuminator use.
Details electrical connection ports on the microscope rear.
Guide for mounting different types of sample stages.
Instructions for removing and attaching the fixed mechanical stage.
Procedures for removing and attaching the rotatable mechanical stage.
Guide for centering the stage for optimal viewing.
How to adjust the stage drive length for better ergonomics.
Instructions for fitting or removing optional drive knob sleeves.
Guide for adjusting the torque of the ergonomic drive knobs.
Instructions for changing the specimen holder on the mechanical stage.
Information about the Pol rotary stage.
Procedure for removing the Pol rotary stage.
Steps for attaching the Pol rotary stage.
Guide for centering the rotary stage for optimal viewing.
Procedure for centering objectives with the rotary stage.
Information on the motorized mechanical stage.
Steps for assembling and connecting the motorized stage with coaxial drive.
Details on connecting the motorized stage via CAN bus.
Guide for assembling the stage and connecting control peripherals.
Instructions for mounting the specimen holder.
Details on connecting the CAN/USB converter for PC communication.
Information on using the docking station with the motorized stand.
Guide for installing the fixed polarizer.
Overview of controls and functions for the coded Axio Imager 2.
Details operations and controls specific to the motorized version.
Instructions for powering the microscope and HAL 100 illuminator on and off.
Guide for switching the HBO 100 illuminator on and off.
Details on the motorized binocular phototube with two camera ports.
Explains the Light Manager function for optimizing illumination settings.
Explains the Contrast Manager for automatic contrast method settings.
Guide to operating the motorized microscope using the TFT touchscreen.
Describes the main areas of the TFT display screen layout.
Details the function of navigation buttons on the TFT display.
Explains the status line providing information on current settings.
Describes the control area where component operations take place.
Outlines the menu structure of the microscope's software interface.
Describes the initial screen displayed after the microscope is started.
How to set and use a favorite page for quick access.
Accessing operation pages like Turret, Illumination, Auto. operation, XYZ.
Details the Revolver page for configuring objectives and other settings.
Details functions for light path and intensity control.
Accessing Soft Keys and HW Settings for automatic operations.
Managing stage position and measurement functions.
Accessing configuration settings for components, extras, and info.
Configuring objectives, reflector turrets, and other components.
Accessing Light Manager, Contrast Manager, and other settings.
Displays firmware version information.
Accessing user selection, configuration, and admin pages.
Adjusting TFT display brightness and turning it on/off.
Guide to capturing large overview images using tiling or MosaiX.
Procedure for relocating saved events on a sample carrier.
Basic settings for capturing overview shots.
Steps for calibrating the microscope stage.
Explains the ACR system and custom component configuration.
Guide for programming rewritable ACR reflector modules.
Details on configuring user-defined filter sets using software.
Information on configuring motorized condensers for reproducible settings.
Overview of illumination and contrast techniques.
Step-by-step guide for setting up Köhler illumination for brightfield.
Procedure for setting up transmitted-light darkfield illumination.
Guide for setting up transmitted-light phase contrast microscopy.
Procedure for setting up transmitted-light DIC contrast.
Setting up transmitted-light polarization for observing crystal optical characteristics.
How to measure path differences using compensators.
Procedure for setting up transmitted-light circular polarization contrast.
Setting up transmitted-light polarization for conoscopic observation of crystals.
Guide for setting up reflected-light brightfield microscopy.
Procedure for setting up reflected-light darkfield microscopy.
Steps for setting up reflected-light DIC and C-DIC contrast.
Procedure for setting up reflected-light TIC technique.
Guide for setting up reflected-light fluorescence microscopy.
Procedure for detecting bireflection and pleochroism in reflected light.
General guidelines for maintaining and cleaning the microscope.
Details on regular maintenance and checks for the instrument.
Routine checks to ensure proper functioning and safety.
Instructions for replacing fuses on the manual stand.
Procedure for changing fuses on the motorized stand's power supply.
Table of common problems, their causes, and solutions.
List of spare parts, consumables, and tools for the microscope.
Information on how to contact ZEISS Service for repairs.
Glossary of abbreviations used throughout the manual.
Alphabetical index of topics covered in the manual for quick reference.
Lists patents and registered utility models related to the instrument.
General| Microscope Type | Upright Microscope |
|---|---|
| Focusing | Coaxial coarse and fine focusing |
| Optical System | Infinity-corrected optical system |
| Illumination | Transmitted Light, Reflected Light (Epi-fluorescence) |
| Light Sources | Halogen, LED, or mercury vapor |
| Objective Lenses | Plan-Apochromat, Achroplan, EC Plan-Neofluar |
| Stage | Motorized or manual stage options |
| Focus Drive | Motorized or manual focus drive |
| Camera Compatibility | Compatible with various digital cameras |
| Camera Ports | C-mount |
| Software | ZEN software |
| Software Compatibility | Windows |
| Contrast Methods | Brightfield, Darkfield, Phase Contrast, DIC, Fluorescence |
| Modularity | Highly modular system |
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