Zeiss Axio Imager 2 - Capturing Large Overviews (Tiles or MosaiX Images) on Axio Imager 2

ZEISS OPERATION Axio Imager 2
Capturing large overviews (tiles or MosaiX images) on Axio Imager 2
154 430000-7544-001 01/2016
4.9 Capturing large overviews (tiles or MosaiX images) on Axio Imager 2
Preparatory steps:
1. Insert a reticle in an eyepiece and mount the eyepiece (see Section 3.5 on page 36 f).
2. Assemble and turn on the stage in accordance with instructions.
3. Insert the mounting frame into a 3-plate stage or apply a scanning stage or an object guide to a 2-
plate stage or CAN bus stage and manual mechanical stage (see Section 3.35 from page 70).
4. Insert the calibrating specimen and focus along the x-axis (parallel line of the specimen).
5. Adjust the stage so that the specimen holder runs exactly parallel to the stand.
- Unscrew the connecting bolts from the stage on the stage carrier,
- Align the stage or
- Adjust the specimen holder.
6. Adjust the mounting frame (if applicable), to minimize the focusing operation of the specimen on the
x and y axis.
7. Orient the camera to the center of the beam path to center the illumination.
- Search for a reference object on the calibrating specimen (cross-hairs or similar) which is then
superimposed on the crosshairs of the inserted reticle in the eyepiece using the stage control
(joystick, coax drive or trackball).
- Move the reference object to the center of the optical field of view and focus.
- Activate crosshairs in the live camera image on the computer monitor and, if necessary, focus the
camera image using an adjustable camera adapter.
- Turn the adjusting screws for the x and y axis of the adapter until the crosshairs of the reference
object in the field of view (microscope) are overlaid with the crosshairs in the live image (camera)
on the screen.
8. Set the axis orientation in the MTB 2011 config.exe and in the AxioVision or ZEN (blue edition)
imaging software.
9. Set the camera image orientation.
The orientation and amalgamation of the final image on the screen should correspond to the position
of the specimen on the specimen stage (e.g. label to the left).
Settings must be made for the last two items
of the stage / stage controller used. These are
shown in the following table.