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Zeiss Axio Observer Series User Manual

Zeiss Axio Observer Series
192 pages
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Axio Observer
Inverted microscope
Operating manual

Table of Contents

Other manuals for Zeiss Axio Observer Series

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Zeiss Axio Observer Series Specifications

General IconGeneral
Camera InterfaceC-Mount
SoftwareZEN Imaging Software
Imaging TechniquesBrightfield, Darkfield, Phase Contrast, DIC, Fluorescence
StageMotorized XY stage
FocusMotorized focus drive
Optical SystemInfinity Optical System
Magnification Range5x to 100x
IlluminationLED or Halogen light source
Camera PortsSide and top ports available
ApplicationsLive cell imaging
Objective MountMotorized or Manual Objective Turret (6- or 7-position, model dependent)

Summary

1 Introduction

2 Safety Instructions

2.1 General Information

General safety instructions and warnings for operating the microscope and handling its components.

2.2 Warranty information

Details on the manufacturer's warranty, exclusions, and liability for misuse or unauthorized repairs.

2.3 Warning and information labels on the device

Explanation of warning and information labels found on the microscope and its accessories.

3 Description of the Device

3.1 Designation and intended use

Defines the Axio Observer microscope models and their primary applications in microscopy.

3.2 Instrument description and main features

Overview of the microscope's stand fittings, accessory components, and key technical features.

3.3 Equipment and compatibility table

Detailed table showing equipment options and their compatibility across different Axio Observer models.

3.4 System overview Axio Observer Bio / Med

Diagrammatic overview of system components for Axio Observer Bio / Med microscope configurations.

3.5 System overview Axio Observer materials (Mat)

Diagrammatic overview of system components for Axio Observer materials microscope configurations.

3.6 Ambient conditions

Specifies permissible ambient temperature, humidity, air pressure, and altitude for operation.

3.7 Technical data

Provides detailed technical specifications including dimensions, weight, operating data, and power requirements.

4 Installation Instructions and First-Time Set-Up

4.1 Installation instructions and space requirements

Details on installation requirements, dimensions, and necessary space for the microscope setup.

4.2 First-time set-up

Describes the services included for initial installation and setup by a ZEISS representative.

4.3 Unpacking and installing the microscope

Instructions for unpacking the microscope and performing the initial physical installation.

4.4 Attaching the binocular (photo) tube

Step-by-step guide for fitting or changing the binocular (photo) tube on the microscope.

4.5 Fitting the carrier for transmitted-light illumination

Procedure for fitting the carrier for transmitted-light illumination, including LCD display connection.

4.6 Fitting the holder with LCD display on 5, 5 materials stand

Instructions for mounting the holder with LCD display on the 5, 5 materials stand.

4.7 Screwing in the objectives

Guidance on correctly and securely screwing objectives into the microscope nosepiece.

4.8 Fitting the Aqua Stop II

Instructions for fitting the Aqua Stop II to protect objectives and nosepiece when working with liquids.

4.9 Fitting the microscope stages

Details on mounting various microscope stages using appropriate spacer disks and frames.

4.10 Condensers

Guidance on attaching and fitting different condenser types to the Axio Observer microscope.

4.11 Reflector turret

Instructions for fitting, loading, and changing components within the reflector turret.

4.12 Fitting the TFT display to the 7, 7 materials stand

Procedure for attaching the TFT display to the 7, 7 materials stand.

4.14 Connectors on the rear of the Axio Observer 3, 3 materials

Diagram and key identifying connectors on the rear of the Axio Observer 3, 3 materials.

4.15 Connectors on the rear of the Axio Observer 5, 5 materials

Diagram and key identifying connectors on the rear of the Axio Observer 5, 5 materials.

4.16 Connectors on the rear of the Axio Observer 7, 7 materials

Diagram and key identifying connectors on the rear of the Axio Observer 7, 7 materials.

4.18 Connecting the microscope to the mains

Instructions for connecting the microscope stand and power supply units to the mains.

4.21 Mounting the microLED illuminator for transmitted and reflected light

Steps for mounting the microLED illuminator for both transmitted and reflected light applications.

4.22 HAL 100 illuminator

Information on fitting and adjusting the HAL 100 illuminator for transmitted and reflected light.

4.23 HBO 100 illuminator

Instructions for fitting and adjusting the HBO 100 illuminator.

5 Operation

5.1 Operating and function controls - overview

Overview of the operating and function controls for different Axio Observer models (3, 5, 7).

5.2 Operating and function controls - description

Detailed description of various controls, buttons, and their functions on the Axio Observer microscope.

5.3 Using objectives

Guidance on selecting and using objectives, including understanding their labeling and magnification.

5.4 Brightfield transmitted light microscopy - quick guide

A quick guide to setting up and performing brightfield transmitted light microscopy.

5.5 Brightfield reflected light microscopy - quick guide

A quick guide to setting up and performing brightfield reflected light microscopy.

5.6 Basic settings on Axio Observer 3, 3 materials stand

Describes basic settings and Light Manager functions for the Axio Observer 3, 3 materials stand.

5.7 Basic settings on Axio Observer 5, 5 materials stand

Details on basic settings, LCD display, and initialization for Axio Observer 5, 5 materials.

5.8 Light Manager on the Axio Observer 3, 3 materials

Explanation of the Light Manager function and its capabilities for Axio Observer 3, 3 materials.

5.9 Light Manager on Axio Observer 5, 5 materials, and 7, 7 materials stands

Details on Light Manager modes (OFF, CLASSIC, SMART) for Axio Observer 5 and 7 stands.

5.10 Contrast Manager on the Axio Observer 7, 7 materials

Functionality of the Contrast Manager for rapid switching and configuring mixed contrasts.

5.11 TFT display touchscreen on the Axio Observer 7, 7 materials

Guide to operating and configuring the microscope using the TFT display touchscreen.

5.12 Illumination and contrast techniques

Detailed procedures for various illumination and contrast techniques like brightfield, phase, and DIC.

5.13 Image orientation of camera outputs for documentation

Information on image orientation and movement for camera outputs via different documentation ports.

5.14 Interface 60N (external thread M52 x 1)

Details on using the 60N interface for camera adaptation on sideports and baseports.

5.15 Photomicrography with SLR camera

Instructions for connecting and using 35 mm SLR cameras for photomicrography.

5.16 Photomicrography using a digital camera and videomicroscopy

Guide for connecting digital cameras and using videomicroscopy with the Axio Observer.

6 Care, Maintenance, Troubleshooting and Service

6.1 Care

Procedures for cleaning and protecting the microscope to ensure optimal performance and longevity.

6.2 Maintenance

Guidelines for regular instrument checks, including power cables, illuminators, and optics.

6.3 Service

Information on who to contact for repairs and authorized service personnel.

7 Appendix

7.1 List of abbreviations

Alphabetical list of abbreviations used throughout the manual with their meanings.

7.2 List of illustrations

A comprehensive list of all figures and illustrations in the manual with their corresponding page numbers.

7.3 Index

An alphabetical index of topics covered in the manual for quick reference.

7.4 Patent rights

List of patents protecting the instruments, components, and methods described in the manual.

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