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Zeiss Axio Observer 5 User Manual

Zeiss Axio Observer 5
192 pages
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Axio Observer
Inverted microscope
Operating manual

Table of Contents

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Zeiss Axio Observer 5 Specifications

General IconGeneral
Optical SystemInfinity Optical System
IlluminationLED
Objective LensesPlan-Apochromat
StageMotorized or Manual Stage
Focus DriveMotorized or Manual Focus
Camera IntegrationCompatible with various cameras
Camera PortC-Mount
Observation TubesBinocular or trinocular tubes with ergonomic design
SoftwareZEN Software
AutomationFully Automated Options Available
Contrast MethodsPhase Contrast, DIC, Fluorescence
DimensionsVaries by configuration
WeightVaries by configuration

Summary

Safety Instructions

Installation Instructions and First-Time Set-Up

First-Time Set-Up Services

Outlines the services provided by ZEISS for initial installation and setup.

Unpacking and Microscope Installation

Provides instructions for safely unpacking and preparing the microscope for setup.

Power Supply and Microscope Connection

Connecting to Mains Power

Instructions on how to connect the microscope to the main power supply.

Illuminator Mounting and Disconnection

Disconnecting from Mains Supply

Procedures for safely disconnecting the microscope from the mains power supply.

Operation

Light Manager Functionality

Light Manager on Axio Observer 3

Explains the functions and features of the Light Manager for the Axio Observer 3.

Light Manager on Axio Observer 5 & 7

Details the purpose, modes, and functions of the Light Manager for Axio Observer 5 and 7.

TFT Display Touchscreen Operation

Microscope Control Functions

Objective and Reflector Configuration

Information on configuring objectives, reflectors, and related optical components.

Settings and Configuration

Illumination and Contrast Techniques

Transmitted Light Brightfield (Köhler)

Comprehensive guide to setting up transmitted light brightfield illumination using Köhler principles.

Transmitted Light Phase Contrast

Explains the phase contrast technique and its principles for examining unstained specimens.

Transmitted Light DIC

Guide on setting up DIC for transmitted light to produce high-contrast, 3D images.

Transmitted Light PlasDIC Contrast

Explains PlasDIC contrast for relief-like images, useful for thicker specimens.

Reflected Light Techniques

Setting Reflected Light Brightfield (Köhler)

Guide for setting up reflected light brightfield microscopy using Köhler illumination.

Setting Reflected Light Fluorescence Contrast

Explains reflected light fluorescence technique for high-contrast images of fluorescent substances.

Setting Reflected Light Polarization

Guide for setting up reflected light polarization for analyzing anisotropic samples.

Setting Reflected Light Darkfield

Guide for setting up reflected light darkfield to examine specimens with surface deviations.

Setting Reflected Light DIC and C-DIC

Instructions for setting up reflected light DIC and C-DIC for high-contrast images.

Setting Reflected Light TIC

Guide for setting up reflected light TIC for imaging and measuring object structures.

Photomicrography Methods

Care, Maintenance, Troubleshooting, and Service

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