Accumax SMART - Procedure to Check Calibration; Conversion of Weight Readings to Volume; Calculation for In-accuracy (Systematic Error); Calculation for Impression (Random Error)

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SMART PIPETTE - USER GUIDE
Conversion of weight readings to volume
Xi = Balance Reading
n = number or reading
Z = Conversion factor
[example Z=1.0040 ul/mg at 25°c and 1013 hPa]
Mean Value V = X · Z
__
MeanValueX=∑ Xi
n
_
Calculation for in-accuracy (Systematic Error)
_
V = Mean Value
Vo = Particular volume at which readings are taken
A% = V - Vo
_
Vo
· 100
Procedure to check calibration
The pipette is checked at maximum volume, at 50% of maximum
volume and at minimum or 10% of maximum volume, whichever is
higher.
•A new tip is first pre-wetted 3-5 times and a series of ten pipetting
is done at each volume.
•Use of forward pipetting technique is recommended.
•Calculate the inaccuracy and imprecision for all three volumes as
per EN ISO 8655/ DIN 12650 standards on the basis of the following
calculation
Calculation for Impression (Random Error)
CV% = 100 · S
_
V
S = Standard Deviation
V = Mean Value
n = number of readings
S=
i=1
∑(Vi-V)
2
n
n - 1
•Compare the results to the limits in the subsequent tables