54 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
3
Using the Pump
Best Practices
Best Practices
Daily / Weekly tasks
Daily tasks
• Replace mobile phase based on water/buffer.
• Replace organic mobile phase latest every second day.
• Check seal wash solvent.
Weekly tasks
• Change seal wash solvent (10 % / 90 % isopropanol/water) and bottle.
• Flush all channels with water at 2.5 – 3 mL/min for 5 min to remove salt
deposits if buffer applications were used.
• Inspect solvent filters for dirt or blockages. Clean or exchange if no flow is
coming out of the solvent line when removed from the degasser inlet.
Power up / Shut-down the pump
Power up the pump
• Use new or different mobile phase (as required).
• Purge pump heads with 2.5 – 3 mL/min for 5 min.
• Stabilize the system by running for 10 – 20 min.
Long-term shut-down of the system
• Flush system with water to remove buffer.
• Remove all samples from the sampler and store according to good
laboratory practice.
• Use recommended solvents to store the system.
• Power off the system.
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