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Agilent Technologies LC Series User Manual

Agilent Technologies LC Series
262 pages
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Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II Isocratic-
and Quaternary Pumps
User Manual

Table of Contents

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Agilent Technologies LC Series Specifications

General IconGeneral
BrandAgilent Technologies
ModelLC Series
CategoryWater Pump
LanguageEnglish

Summary

1 Introduction

Introduction to the Pump

Provides an overview of the pump, instrument, and connectors.

G7110B Isocratic Pump

Details the Agilent 1260 Infinity II Isocratic Pump, its features, and product description.

G7111A Quaternary Pump VL

Covers the Agilent 1260 Infinity II Quaternary Pump VL, product description, and features.

G7111B Quaternary Pump

Describes the Agilent 1260 Infinity II Quaternary Pump, its product description, and features.

G5654A Bio-inert Pump

Details the Agilent 1260 Infinity II Bio-inert Pump, its product description, and features.

Overview of the Hydraulic Path

Explains the low pressure mixing pumps and their hydraulic path for quaternary and bio-inert pumps.

Leak and Waste Handling

Discusses the design for safe leak and waste handling, including solvent cabinet and leak plane outlets.

2 Site Requirements and Specifications

Site Requirements

Outlines environmental requirements for optimal instrument performance, including power and bench space.

Physical Specifications

Provides physical specifications for the Isocratic, Quaternary VL, Quaternary, and Bio-inert pumps.

Performance Specifications

Details performance specifications for the Agilent 1260 Infinity II Isocratic Pump.

3 Using the Pump

Turn on/off

Explains the procedure for turning the instrument on and off using the power switch and control software.

Status Indicators

Describes the meaning of the module status indicator and its six possible conditions.

Best Practices

Offers advice on daily/weekly tasks and powering up/shutting down the pump for optimal operation.

Prepare the pump

Details the steps for purging the pump and the use of seal wash for pump maintenance.

How to deal with solvents

Provides recommendations for handling solvents, including bottle labeling and filter use.

Leak and Waste Handling

Discusses safe handling of solvents, samples, and reagents, including waste container requirements.

Hints for Successful Use of the Pump

Offers tips for successful pump operation, including solvent cabinet placement and purge valve frit checks.

Setting up the Pump with the G4208A Instant Pilot

Guides on generic operation and setting up module-specific parameters using the G4208A Instant Pilot.

Setting up the Pump with the Instrument Control Interface

Explains how to set up the pump using the instrument control interface, including configuration and signals.

Solvent Information

Provides recommendations on solvent usage, filtering, and avoiding corrosion.

Materials in Flow Path

Lists materials used in the flow path for different pump models, detailing their specifications.

Bio-inert Materials

Describes the high-quality materials used in the Bio-inert LC system for optimum inertness.

Material Information

Details material properties and compatibility with solvents, including PEEK, Polyimide, PE, Tantalum, Stainless Steel, and Titanium.

Algae Growth in HPLC Systems

Explains the problems caused by algae in HPLC systems and how to prevent them.

Prevent Blocking of Solvent Filters

Offers suggestions to prolong solvent filter lifetime and maintain module performance.

Normal Phase Applications

Discusses the use of specific valves and seals for normal phase applications and potential issues.

4 Optimizing Performance

Using the Degasser

Explains the importance of the built-in degasser in the quaternary pump and its inclusion in the flow path.

Operational Hints for the Vacuum Degasser

Provides guidance on priming the vacuum degasser for first-time use or after extended periods of inactivity.

Operational Hints for the Multi Channel Gradient Valve (MCGV)

Offers advice on using the MCGV with salt solutions and organic solvents to avoid precipitation and ensure performance.

When to use the Seal Wash Function

Explains when the seal wash function is recommended, particularly with concentrated buffer solutions.

Choosing the Right Pump Seals

Guides on selecting appropriate pump seals for different applications, including normal phase solvents.

Optimize the Compressibility Compensation Setting

Details how to optimize the compressibility compensation setting for best flow accuracy and mixing performance.

5 Troubleshooting and Diagnostics

User Interfaces

Describes the available tests that vary depending on the user interface used.

Agilent Lab Advisor Software

Explains the Agilent Lab Advisor Software's capabilities for managing labs and performing diagnostics.

6 Error Information

What Are Error Messages

Explains when and how error messages are displayed, including their impact on module operation.

General Error Messages

Lists and describes common error messages applicable to all Agilent HPLC modules, such as Timeout and Shutdown.

Module Error Messages

Details pump-specific error messages, including Solvent Zero Counter and Pressure errors.

7 Test Functions and Calibration

System Pressure Test

Explains the System Pressure Test for checking LC system tightness and identifying leaks.

Leak Rate Test

Describes the Leak Rate Test for verifying internal pump tightness and identifying leak sources.

8 Maintenance

Introduction to Maintenance and Repair

Introduces the module's design for easy repair, focusing on front-accessible maintenance.

Warnings and Cautions

Lists critical warnings and cautions related to toxic solvents, electrical shock, and personal injury during maintenance.

Overview of Maintenance and Repair

Outlines simple repair procedures that can be performed without opening the main cover.

Cleaning the Module

Provides instructions for cleaning the module case using a soft cloth and water or mild detergent.

Remove and Install Doors

Details the procedure for removing and installing the front doors of the module.

Checking and Replacing the Solvent Filter

Explains the importance of a functional solvent filter and how to check and replace it.

Exchange the Passive Inlet Valve

Guides on exchanging the passive inlet valve if it is internally leaking or showing backflow.

Exchange the Outlet Valve

Provides instructions for exchanging the outlet valve if it is internally leaking.

Exchange the Purge Valve Frit or the Purge Valve

Describes how to exchange the purge valve frit or the entire purge valve if contaminated or leaking.

Replace the O-Ring on the Purge Valve

Details the procedure for replacing the O-ring on the purge valve when it is damaged.

Remove the Pump Head Assembly

Explains how to remove the pump head assembly for seal or piston exchange.

Maintenance of a Pump Head Without Seal Wash Option

Covers maintenance procedures for pump heads that do not have a seal wash option.

Maintenance of a Pump Head with Seal Wash Option

Details maintenance procedures for pump heads equipped with a seal wash option.

Reinstall the Pump Head Assembly

Guides on reinstalling the pump head assembly after maintenance or component replacement.

Seal Wear-in Procedure

Outlines the seal wear-in procedure, including solvent usage and pressure settings.

Exchange the Multi-Channel Gradient Valve (MCGV)

Provides instructions for exchanging the MCGV, emphasizing regular flushing to prevent issues.

Replace the Solvent Selection Valve (Isocratic Pump)

Details how to replace the solvent selection valve in the isocratic pump if it is leaking or blocked.

Exchange the Active Inlet Valve (AIV) or its Cartridge

Guides on exchanging the active inlet valve or its cartridge if it is internally leaking.

Exchange the Seal Wash Cartridge

Explains how to exchange the seal wash cartridge, including removing and installing the peristaltic pump.

Replace Leak Handling System Parts

Describes how to replace parts of the leak handling system, such as the leak adapter and tubing.

Replacing the Module Firmware

Provides instructions on upgrading or downgrading the module's firmware using Agilent Lab Advisor software.

9 Parts for Maintenance

Accessory Kits

Lists the items included in the Accessory Kit G7111-68755 and G5654-68755.

Pump Head Assembly Without Seal Wash

Details the components of the Pump Head 1200 SL without Seal Wash (G1312-60056).

Pump Head Assembly with Seal Wash Option

Lists components for pump head assemblies with seal wash (G1312-60045) and bio-inert versions.

Outlet Valve

Shows the different outlet valve part numbers for various Agilent pump models.

Purge Valve Assembly

Lists the parts included in the Purge Valve Assembly for different pump models.

Active Inlet Valve Assembly

Details the components for the Active Inlet Valve Assembly, including upgrade kits and cartridges.

1260 Infinity II Max Uptime Kit

Lists optional parts for the 1260 Infinity II Max Uptime Kit, sold separately.

1260 Infinity II Max Uptime Kit Bio

Lists optional parts for the 1260 Infinity II Max Uptime Kit Bio, sold separately.

1260 Infinity II Low Dispersion Kit MCT

Lists optional parts for the 1260 Infinity II Low Dispersion Kit MCT.

HPLC System Tool Kit

Shows the contents of the HPLC System Tool Kit, including various tools and adapters.

Solvent Cabinet

Describes the Solvent Cabinet Kit and provides a reference for its usage guideline.

Bottle Head Assembly

Lists the components included in the Bottle Head Assembly (G7120-60007).

Hydraulic Path of the Quaternary Pump

Illustrates the hydraulic flow path for the Quaternary Pump, detailing capillary and tubing connections.

Hydraulic Path of the Isocratic Pump

Illustrates the hydraulic flow path for the Isocratic Pump, detailing capillary and tubing connections.

10 Identifying Cables

Cable Overview

Provides a general overview of available cables, including Analog, Remote, and CAN cables.

Analog Cables

Describes Analog cables, including connections to A/D converters and BNC connectors.

Remote Cables

Details various remote cables, including ERI, ERI-APG, and ERI-APG-RJ45 types with pin assignments.

CAN Cable

Explains CAN and LAN cables used for module-to-module communication and network connectivity.

Agilent Module to PC

Describes RS-232 cables used for connecting Agilent modules to a PC.

Agilent 1200 Module to Printer

Explains how to connect modules to a printer, specifically for USB Flash Drive usage.

11 Hardware Information

Firmware Description

Explains the instrument firmware structure, including resident and main system sections.

Electrical Connections

Details the electrical connections for CAN, ERI, LAN, USB, and power input, including cable usage notes.

Rear View of the Module

Shows a diagram of the module's rear view, highlighting connectors and labels.

Interfaces

Provides a table summarizing the interfaces available on Agilent InfinityLab LC Series modules (CAN, USB, LAN, RS-232, Analog, APG/ERI).

Setting the 6-bit Configuration Switch

Explains the location and function of the 6-bit configuration switch for LAN and initialization settings.

Special Settings

Covers special settings like Boot-Resident/Main modes and Forced Cold Start for firmware updates or parameter resets.

Instrument Layout

Describes the industrial design and packaging concept of the module, focusing on E-PAC.

Early Maintenance Feedback

Explains the EMF feature for monitoring component usage and providing maintenance feedback.

12 LAN Configuration

What You Have to Do First

Outlines initial steps for LAN configuration, including noting the MAC address and connecting the interface.

TCP/IP parameter configuration

Explains the necessity of configuring TCP/IP parameters (IP address, Subnet Mask, Gateway) for network operation.

Configuration Switches

Describes the location and function of the configuration switches for LAN and initialization modes.

Initialization Mode Selection

Details the selectable initialization modes (Default IP, Stored IP, DHCP) and their principles.

Dynamic Host Configuration Protocol (DHCP)

Provides general information about DHCP and its use for automatic IP parameter configuration.

Manual Configuration

Explains how to manually configure parameters stored in non-volatile memory, including using Telnet.

13 Appendix

General Safety Information

Covers essential safety precautions for operating, servicing, and repairing the instrument.

Solvents

Warns about toxic, flammable, and hazardous solvents and provides handling precautions.

Safety Symbols

Explains various safety symbols used on the apparatus and their meanings.

Waste Electrical and Electronic Equipment (WEE) Directive

States product compliance with the European WEEE Directive and disposal requirements.

Radio Interference

Discusses screened cables for radio interference protection and compliance with EMC regulations.

Sound Emission

Provides the manufacturer's declaration regarding sound pressure emission levels.

Agilent Technologies on Internet

Directs users to the Agilent website for the latest product and service information.

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