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Agilent Technologies 1290 Infinity II 2D-LC

Agilent Technologies 1290 Infinity II 2D-LC
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Agilent 1290 Infinity II 2D-LC Solution
MassHunter Acquisition for TOF and Q-TOF
User Guide
2D-LC User Guide

Table of Contents

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Agilent Technologies 1290 Infinity II 2D-LC Specifications

General IconGeneral
Type2D-LC System
Data RateUp to 240 Hz
Pump TypeBinary Pump
Injection Volume Range0.1-100 µL
Detector TypeDiode Array Detector (DAD)
Wavelength Range190-950 nm
2D-LC CapabilityYes
CompatibilityAgilent OpenLAB CDS, Agilent MassHunter Workstation
Detection TechnologyUV-Vis
Maximum Pressure1300 bar

Summary

Introduction

Product Description

Describes the 1290 Infinity II 2D-LC System, its applications, and benefits.

Features

Lists key features of the Agilent InfinityLab 2D-LC Solutions.

Terms related to 2 D-LC

Defines essential terms used in 2D-LC chromatography.

Concepts of 2 D-LC

Concepts of 2 D-LC

Explains the fundamental concepts and components of a 2D-LC system.

Heart-Cutting 2 D-LC (LC-LC)

Describes the characteristics and principles of Heart-Cutting 2D-LC.

Multiple Heart-Cutting and High Resolution Sampling 2 D-LC

Explains the Multiple Heart-Cutting and High Resolution Sampling techniques.

Comprehensive 2 D-LC (LCxLC)

Details the principles and characteristics of Comprehensive 2D-LC.

Active Solvent Modulation (ASM)

Introduces Active Solvent Modulation and its benefits for 2D-LC.

Compatibility Matrix

Supported Chromatographic Data Systems

Lists recommended MassHunter Workstation Data Acquisition revisions.

Supported Drivers

Details the supported firmware, MassHunter, and LC/CE driver versions.

Supported Operating Systems

Specifies the operating systems compatible with MassHunter CDS.

Supported Firmware

Lists Agilent 2D-LC Software tested firmware revisions.

PC Requirements

Outlines the recommended PC specifications for MassHunter Workstation.

Installation

Hardware Installation

Covers hardware setup, including delivery checklist and valve configurations.

Software Installation

Provides step-by-step instructions for installing the 2D-LC software.

2 D-LC Data Acquisition in MassHunter Workstation 11

Start the Data Acquisition Software

Guides users on how to start the MassHunter Data Acquisition software.

Overview 2 D-LC in MassHunter Acquisition 11

Describes the dashboard as the common UI for instrument control.

Instrument Status

Shows the current status of each configured device in the 2D-LC solution.

2 D-LC User Interface

Explains the 2D-LC device pane and its functionalities.

Method Editor Window

Details how to enter acquisition parameters for the method.

Method Parameters

Method Editor 2 D-LC

Describes the method setup dialog for editing 2D-LC specific parameters.

Set the 2 D-LC Method parameters

Explains how setting the 2D-LC operation mode affects the system.

Edit the Sampling Table

Details how to specify when the selected 2D-LC mode is active.

Define Parameters for Peak-Based Heart Cut

Configures parameters for MHC and HiRes peak-based operations.

Define the 2 D Gradient

Guides on optimizing the gradient method for the second dimension run.

Use Loop Flushing and Active Solvent Modulation (ASM)

Details how to find optimal dilution of 1D solvents using ASM.

Use Peak Trigger

Explains how to set peak trigger in time-based and peak-based modes.

Method Development of Active Solvent Modulation (ASM)

Method Development of Active Solvent Modulation (ASM)

Helps find optimal dilution of 1D solvents for best 2D resolution.

Method Parameters

Explains switching ASM functionality on and off.

Optimize the Dilution by Using ASM Capillaries

Discusses selecting different ASM capillaries for optimal results.

Include the ASM Phase to the 2 D Gradient

Explains how the ASM phase shifts the analytical gradient start.

Run the System

Familiarization to Start a System Run

Illustrates 2D-LC capabilities and guides user to start methods.

Prepare the 2 D-LC System

Provides steps for preparing the system for LC and MS analysis.

Configure the 2 D-LC System

Details requirements for the driver-based 2D-LC solution.

Configure 2 D-LC Hardware

Focuses on 2D-LC valves and capillary connections.

Checkout Familiarization Procedure

Outlines the requirements for the checkout familiarization procedure.

Run the Experiment

Provides recommended conditions for standard heart-cutting experiments.

Data Analysis

2 D-LC Data AnalysisData Evaluation for MassHunter

Information on analyzing 2D-LC data using MassHunter software.

Automated File Splitting

Enables automatic generation of correct cutting sequences.

MassHunter Qualitative Analysis Software

Works with 2D data for compound identification and analysis.

Workflow 1 D UV Data Extraction - Alternative 1

Provides steps for extracting UV data from chromatograms.

Workflow 2 D MS Data

Explains how to work with 2D MS data.

Troubleshooting and Diagnostics

Agilent Lab Advisor Software

Standalone product for lab management and system overview.

Lab Advisor Instrument Control

Control of 2D pump and 2D-LC valves.

2 D-LC Hardware License Handling

Procedures for USB hardware dongle installation/deinstallation.

Lab Advisor Service & Diagnostic

Tools for declustering and running diagnostic tests.

Pump Head Leak Test for the 2 D Pump

Determines leakage of individual pump heads.

System Pressure Test for the 2 D Pump

Determines leak tightness of the system between pump and blank nut.

The Basic Principle of Troubleshooting

Explains a step-by-step approach to 2D-LC chromatography problems.

Error Information

What Are Error Messages

Explains the meaning and causes of error messages.

General Error Messages

Lists generic error messages common to Agilent HPLC modules.

Timeout

Details the 'Timeout' error (Error ID: 0062).

Leak Sensor Short

Describes the 'Leak Sensor Short' error (Error ID: 0082).

Module-Specific Error Messages

Lists specific error messages for different modules.

Initialization of Valve Failed

Describes the 'Initialization of Valve Failed' error (Error ID: 24000).

Maintenance

Introduction to Maintenance

Highlights the design for easy maintenance of the 2D-LC solution.

Warnings and Cautions

Provides essential safety warnings for operation and repair.

Cleaning the Module

Details the procedure for cleaning the module case.

Correcting Leaks

Provides steps for correcting leaks in the system.

Replace Valve Heads

Explains how to install and exchange valve heads.

Replacing the Fuses of the Infinity Valve Drive

Provides instructions for replacing fuses in the valve drive.

Parts for Maintenance

2 D-LC Loops

Lists 2D-LC loops for standard and MHC valve configurations.

2 D-LC Capillaries

Lists capillary kits for 1200 Infinity Series and InfinityLab legacy.

ASM Capillaries

Lists ASM valve capillary replacement kits.

Pressure Release Kit

Details the parts included in the pressure release kit.

Valve Drive Parts

Lists replacement parts for the valve drive.

Valve Head Parts

Illustrates the replacement parts for valve heads.

Valve Options Overview (for 2 D-LC)

Lists available valve options compatible with 2D-LC.

MS Diverter Valve

Lists parts for the MS diverter valve setup.

Accessories and Consumables (for G7116 B)

Details the accessory kit for G7116B.

Theoretical Background

Theoretical basis of 2 D-LC

Explains the principles of 2D-LC separation.

Orthogonality

Defines and discusses orthogonality in 2D-LC separations.

Resolution

Discusses factors affecting chromatographic separation optimization.

Peak Capacity

Defines and explains peak capacity calculation in 2D-LC.

Solvent Elution Modes

Discusses common gradient, shifted, isocratic, and advancing isocratic modes.

Appendix

General Safety Information

General safety precautions for instrument operation, service, and repair.

Before Applying Power

Lists precautions to be taken before applying power to the instrument.

Ground the Instrument

Provides instructions for proper electrical grounding of the instrument.

Solvents

Offers recommendations and warnings for solvent usage.

Waste Electrical and Electronic Equipment (WEEE) Directive

Provides information on proper disposal of electronic waste.

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