14 Theoretical Background
Theoretical basis of 2D-LC
2D-LC User Guide 364
The following different methods of 2D-LC exist:
• Heartcutting (LC-LC)
Only interesting portion of the first dimension effluent transferred to the
second dimension.
• Comprehensive (LCxLC)
Entirety of first dimension effluent sequentially transferred to the second
dimension.
Orthogonality
The 2D-LC separation power depends the fact that the two selectivity
mechanisms in the different separation stages must be as different as possible.
If the mechanisms are completely different and independent the two separations
are called orthogonal.
Any correlation between the selectivity mechanisms degrades orthogonality and
reduces the efficiency of the 2D-LC system.
For strategies to achieve maximum orthogonality, refer to Table 45 on page 373
and Table 46 on page 378.
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