14 Theoretical Background
Solvent Elution Modes
2D-LC User Guide 378
All modes are easily available with the Agilent 2D-LC Acquisition software.
Each mode has advantages and disadvantages. No single mode is superior in all
applications of 2D-LC.
Table 46 Different elution modes in the
2
D (pros and conts)
Criterion Gradient/Shifted gradient Isocratic/Advancing isocratic
Peak capacity Superior Inferior
Diversity of samples
(complex samples)
Superior Inferior
Baseline performance
(sensitivity
Inferior
(baseline drift caused by solvent
gradient)
Superior
Pressure stress
(column lifetime!)
Inferior
(large changes within every 2nd
dimension gradient
Superior
(no pressure changes with
isocratic, gradually changing with
advancing isocratic)
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