7 Method Development of Active Solvent Modulation (ASM)
Method Development of Active Solvent Modulation (ASM)
2D-LC User Guide 186
Method Development of Active Solvent
Modulation (ASM)
ASM method development helps finding the optimal dilution of 1D solvents in the
sample loop for best 2D resolution at lowest cycle time.
After switching on the ASM functionality (see “Method Parameters” on page 187),
execute the steps in the following order:
• “Optimize the Dilution by Using ASM Capillaries” on page 188
• “Optimize the Sample Loop Flush” on page 189
• “Include the ASM Phase to the 2D Gradient” on page 190
• “Optimize Dilution Through Method Settings” on page 191
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