2D-LC User Guide 125
6Method Parameters
Method Editor 2D-LC 126
Set the 2D-LC Method parameters 127
2D LC Operation Mode 127
Define the 1D Pump FLow 129
Define the 2D Pump Flow 129
Define the 2D Solvent 130
Define the Stoptime 130
Define the Posttime 132
Edit the Sampling Table 133
Define the 2D Gradient 139
Use the Flush Gradient 147
Use Peak Trigger 148
Use the Advanced 2D Pump Settings 152
Preview (2D-LC) 154
Further Graphical Explanation 162
Set up a Peak-Based Experiment Graphically 168
Setup 2D Gradient Graphically 173
Setup Second Dimension Gradient with the Graphical User Interface 175
Additional Information 177
Multi-Inject 177
Dynamic Peak Parking 179
This chapter provides background information on method parameters. It helps to
optimize methods in Agilent 1290 Infinity II 2D-LC Solution in the modes standard
heart-cutting, multiple heart-cutting, high resolution sampling and
comprehensive 2D-LC.