2D-LC User Guide 127
6 Method Parameters
Set the 2D-LC Method parameters
Set the 2D-LC Method parameters
2D LC Operation Mode
Setting the mode has the following consequences:
Heart-Cutting (LC-LC)
The Heart-Cutting mode covers two 2D-LC applications Heart Cutting (LC-LC)
and High-Resolution Sampling (HiRes). Once you have selected the Heart-Cutting
mode, you can later define in the software whether you want to use one or the
other mode or even both together.
In Heart-Cutting modus, a relevant volume of
1
D is cut off and injected onto the
2
D column using the
2
D pump. A peak trigger or a time window defines the
volume to be injected on the
2
D column. When heart-cutting starts, a loop is filled
with the peak of interest. Then the injection on the
2
D starts running the gradient
of the
2
D pump.
For details Setting this mode, see “Heart-Cutting 2D-LC (LC-LC)” on page 18.
In contrast to Heart-Cutting, which uses the continuous flowthrough principle, in
high-resolution sampling (HiRes) the Multiple Heart-Cutting (MHC) valve is
switched before and after parking the peak.
When setting up the experiments, keep the following general considerations in
mind:
• Each loop for consecutive snips stores the same sample volume.
• First and last loop cannot be used for parking.
• Solvent transfer from
1
D to
2
D can be reduced.
• Cut number 5 cannot be parked entirely in the Sample Loop. Otherwise cut 6
would got partially to the transfer capillary and would therefore be lost or spoil
cut 5. Cut 5 stays partially in the transfer line and is immediately being
analyzed in
2
D.
• For parking cut 6 in the Sample Loop, the cut first needs to be moved from the
2D-LC Valve to the deck valve. This new volume must be defined in the
configuration of the 2D-LC system.
For details Setting this mode, see “High Resolution Sampling - Peak Parking
Principles” on page 25.
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