6 Method Parameters
Set the 2D-LC Method parameters
2D-LC User Guide 128
Comprehensive 2D-LC (LC*LC)
If you have selected comprehensive 2D-LC, the entire volume of the
1
D will be
injected (using the
2
D pump) onto the
2
D column. Two identical loops are used
alternating, while one loop is filled in
1
D, the volume of the other loop is separated
with the
2
D column.
The Modulation time reflects the duration of one injection cycle in the
2
D. After
that time, the solvent composition gradient will be repeated. The parameter
Modulation time is only used in the Comprehensive mode. The
2
D Gradient stop
time reflects the maximal duration of the gradient in
2
D; the smallest value is
0.01 min. After that time, the Percent B value before the gradient (or the timetable
entry at time = 0.0) is restored. In the Comprehensive 2D-LC mode, the gradient
stops latest when the Modulation time is reached.
Off
Setting the mode OFF then the 2D-LC functionality is disabled. The 2D-LC
instrument is used as a standard 1D-LC instrument that allows you to carry out a
1
D run.
If you load
1
D methods and the 2D-LC mode is still active, you must switch off
the 2D-LC functionality manually.
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