7 Method Development of Active Solvent Modulation (ASM)
Optimize the Dilution by Using ASM Capillaries
2D-LC User Guide 188
Optimize the Dilution by Using ASM Capillaries
A choice of four different ASM capillaries is available for achieving best results.
Longer capillaries reduce, shorter capillaries increase the dilution of 1D solvent in
the sample loop. Install and configure different ASM capillaries (see “Configure
the ASM Valve” on page 190) for optimizing the results.
Capillary p/n Length (mm) Inner diameter
(mm)
Volume
(µl)
ASM factor Split ratio
(loop:ASM)
5500-1300 85 0.12 0.96 5 1:4
5500-1301 170 0.12 1.9 3 1:2
5500-1302 340 0.12 3.8 2 1:1
5500-1303 680 0.12 7.7 1.5 1:0.5