8 Run the System
Prepare the 2D-LC System
2D-LC User Guide 194
Prepare the 2D-LC System
Prepare the 2D-LC System for LC
As a user guide for good preparation, refer to the help instruction and
suggestions of Good Laboratory Practice for HPLC.
1 Condition your Agilent HPLC instrument to have a stable system.
2 For further details, see Best Practices for Using an Agilent LC System
(SD-29000194 Rev. B), or the user manual of each module.
Prepare the 2D-LC System for MS
The ion source parameters depend on the composition and flow rate of the
mobile phase being used. Therefore it is usually worth retuning the mass
spectrometer, after the LC conditions have been determined.
1 Perform an Auto Tune.
2 To clean the source and flush the LC/fluidics lines out prior to starting your
experiment, use LC-MS grade solvents and reagents.
This measure ensures that the optimum sensitivity is achieved, improves
reproducibility, and avoids many common problems.
3 Check additional parameters like the Source Temperature Drying Gas
Temperature and Gas Flow.
Such parameters are rarely adjustable during the analysis and should be
optimized before starting the analysis.
4 For further LC-MS details, see the Q-TOF Acquisition Manual (G3335-90210),
or the module user manual.
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