6 Method Parameters
Set the 2D-LC Method parameters
2D-LC User Guide 130
Define the
2
D Solvent
1 Set the percentage of solvent B to any value from 0 – 100 % in steps of
0.01 %.
Figure 69 2D-LC solvent settings
Solvent A always delivers the remaining percentage of volume. If the rate of
solvent B is, for example, set to 20 %, solvent A, following the calculation %A =
100 - %B, automatically is set to 80 %. The name of the selected solvents and
their solvent channels (A1: ... or A2: ... and B1: ... or B2: ...) are shown in the
corresponding text fields.
2 For each solvent, click the down-arrow and select a calibrated solvent from
the drop-down list. You can also enter additional information (for example,
about buffers) in the adjacent field.
Define the Stoptime
The
2
D pump stop time sets a time limit for your 2D-LC measurement. This
means the runtime of the 1D run plus the runtimes of all 2D cuts. After the stop
time, all gradients are stopped and the pump parameters return to their initial
values.
1 To set the stop time, select the radio button and fill in the field Stoptime.
For the driver-based 2D-LC solution, ensure that the stop time is long enough to
include all
2
D analyses. The run time will not be extended automatically when
cuts remain parked.
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