2D-LC User Guide 131
6 Method Parameters
Set the 2D-LC Method parameters
Figure 70 Stoptime and Posttime settings
Figure 71 If the stop time (grey stop time marker, see green arrow in figure) is not sufficient
for a complete
2
D run, a notification triangle pops up
The
2
D pump is the stop time master for the complete 2D-LC system. The stop
times of all other modules in the system must be set to As Pump/ As Injector
except the
1
D pump module that should set the Stop Time Modus As Injector/No
Limit.
If an alert (triangle) pops up in the chromatogram preview you can hover over the
sign to get more details. Most likely the stop time of the
2
D pump is not sufficient
to analyze all
2
D cuts see picture above). For a correct stop time alignment click
on the stop icon or double click the grey stop time marker in the reference
chromatogram. This action will extend the stop time to a valid number.