1Introduction
Features
2D-LC User Guide 12
Features
Agilent InfinityLab 2D-LC Solutions offers following key features:
• Agilent 2D-LC is based on 1290 Infinity II Systems with UHPLC performance,
fast gradients, high sensitivity and excellent robustness
• Dedicated 2D-LC valves use completely symmetric flow paths for
reproducible retention times and peak areas.
• A wide range of modules can be used in both dimensions. Third-party
detectors are supported via UIB II including the sue of compatible detectors
for data analysis
• Powerful Agilent 2D-LC software is available for OpenLab CDS, MassHunter
and ChemStation. Measurements can be set up easily with a few mouse
clicks: Starting with a 1D measurement, choose spots where you want to
increase resolution and draw your second dimension gradient.
• Agilent 2D-LC instrument control is fully automated and eliminates the need
for tedious manual valve programming. Separation in the first and second
dimension are completely independent by using Agilent multiple heart-cutting
valves for highest storage capacity for up to 12 cuts at one time, fast and
parallel analysis.
• High-resolution sampling is available for flexibly analyzing short cuts to broad
peaks while retaining first dimension separation. By analyzing complete
peaks, highly reproducible quantitative measurements can be achieved.
• Multiple heart-cutting and High-resolution sampling can be combined
arbitrarily within one run
• Shifted gradients, which can be edited graphically or numerically, maximize
the available 2D separation space for highest peak capacity and fastest
analysis
• Dedicated flush gradients are available for fast analysis and minimum
carryover
• Cuts can be defined in time-based mode for highly reproducible
measurements or peak-based mode for variable retention times or unknown
samples. Even in peak-based mode, both first and second dimension
detectors provide complete chromatograms.
• Dynamic peak parking combines time- and peak-based approaches for
dealing with shifting first dimension retention times e.g. of
biopharmaceuticals by using reference compounds.
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