2 Concepts of 2D-LC
Multiple Heart-Cutting and High Resolution Sampling 2D-LC
2D-LC User Guide 22
Principles of Heart-cutting 2D-LC
Multiple Heart-Cutting - Principles
Multiple Heart-Cutting - Principles
Multiple Heart-Cutting 2D-LC is a complex workflow, working on a special
algorithm for filling the sample loops and analyzing the stored cuts, based on
different criteria. “Multiple Heart-Cutting - Principles” on page 22 illustrates the
principles of the Multiple Heart-Cutting algorithm, following these principles:
•
2
D analysis is done as soon as possible. As long as the second dimension is
free, any next cut from the first dimension will be always directly transferred
to the second dimension and analysed. This means:
• The first
1
D cut will be always directly analysed in the second dimension.
• If the second dimension is free, when the next
1
D cut is taken, it will also be
directly analysed.
• If the second dimension is occupied, the next
1
D cut will be stored in the next
sample loop.
• If all sample loops in the first dimension are occupied, the peak is lost.
• A peak parking deck will always be completely analysed, before switching to
the other parking deck.
• Before analyzing a new parking deck, a flush gradient is run to avoid
contamination.
• Stored cuts are analysed in backwards order to avoid contamination.
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