14 Theoretical Background
Solvent Elution Modes
2D-LC User Guide 374
Solvent Elution Modes
Table 46 on page 378 focuses on the effects of elution modes for
2
D separation.
The following elution modes for
2
D separation are commonly used:
• Gradient
A standard gradient of solvent A vs. solvent B for the second dimension
separation will be repeated during the complete first dimension separation
Figure 176 Standard gradient mode
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