Agilent Technologies 1290 Infinity II 2D-LC

2D-LC User Guide 375
14 Theoretical Background
Solvent Elution Modes
• Shifted Gradient
From each
2
D separation to the next the start-%B and end-%B values of the
individual
2
D gradients will be increased in a defined way. Additionally, the
gradient span can be increased from each
2
D gradient to the next.
Figure 177 Shifted gradient mode with increase of start-%B