8 Run the System
Run the Experiment
2D-LC User Guide 214
Run the Checkout Familiarization Procedure for
Comprehensive (LCxLC)
To run the checkout, various hardware configurations are possible, see Table 5
on page 56. Not all options can be shown. As example the Table 14 on page 198
is used here.
To achieve optimal sensitivity, in comprehensive mode, especially for LC/MS
applications, the LC flow is often split prior to the mass spectrometer.
The following parameters have been optimized for this standard configuration.
Parameters can deviate slightly for your system. Run the experiment and
subsequently edit or optimize the method for your setup.
Table 24 Example for a MS passive splitter setup (ratio 1:2)
Description (PN) Usage
TEE, ST, 1/16 inch, Low Dead Volume
(0100-0969)
T-piece
DAD Heat Exchanger Capillary 310 mm, 0.12 mm
i.d. (G1315-87339)
2D detector connected to T-piece
Capillary ST 0.075 mm x 500 mm, long socket
(5500-1205)
Inlet of the LCMS source connected to the other
end of the T-piece
Capillary ST 0.075 mm x 250 mm, long socket
(5500-1206)
Remaining connection to the T-piece is used as
waste capillary
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