Agilent Technologies 1290 Infinity II 2D-LC User Manual

2D-LC User Guide 215
8 Run the System
Run the Experiment
Table 25 Recommended conditions in 1D (HPLC) for comprehensive 2D-LC
Parameter Value
1
D Column Compartment (MCT)
Column RRHD SB-C18, 2.1x 100 mm, 1.8 µm, 1200 bar (858700-902)
Column temperature 40 °C
Stop time As pump/No limit
1
D Pump
Mobile Phase A Water + 0.2 % formic acid
Mobile Phase B Methanol
Flow Rate 0.1 mL/min
Stop time 40 min
Post time 10 min
Mobile Phase
Gradient:
40 % B 0.00 min
60 % B 34 min
90 % B 34.5 min
Autosampler
Injection Volume 2 µLfor Standard LC
0.5 µL Positive Mode for LCMS
10 µL Negative Mode for LCMS
Injection Needle Wash In Flush Port, 10 s, acetonitrile/water (50/50)
Stop time As pump/No limit
1
D Detector (DAD)
Diode-array Detector
Signal A
254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 20 Hz
Stop time Stop time As pump/No limit

Agilent Technologies 1290 Infinity II 2D-LC Specifications

General

Brand	Agilent Technologies
Model	1290 Infinity II 2D-LC
Category	Measuring Instruments
Language	English