2D-LC User Guide 201
8 Run the System
Run the Experiment
Run the Experiment
Run the Checkout Familiarization Procedure for
Standard Heart-Cutting 2D-LC (LC-LC)
To run the checkout, various hardware configurations are possible, see Table 5
on page 56. Not all options can be shown. As example the Table 14 on page 198
is used here.
The following parameters have been optimized for this standard configuration.
Parameters can deviate slightly for your system. Run the experiment and
subsequently edit or optimize the method for your setup.
Table 15 Recommended conditions in 1D (HPLC) for SHC, MHC, and HiRes 2D-LC
Parameter Value
1
D Column Compartment (MCT)
Column RRHD SB-C18, 2.1x 100 mm, 1.8 µm, 1200 bar (858700-902)
Column temperature 40 °C
Stop time As pump/No limit
1
D Pump
Mobile Phase A Water + 0.2 % formic acid
Mobile Phase B Methanol
Flow Rate 0.6 mL/min
Post time 6 min
Mobile Phase
Gradient:
20 % B 0.00 min
100 % B 50 min
Autosampler
Injection Volume 2 µLfor Standard LC
0.5 µL Positive Mode for LCMS
10 µL Negative Mode for LCMS
Injection Needle Wash In Flush Port, 10 s, acetonitrile/water (50/50)
Stop time As pump/No limit
1
D Detector (DAD)
Diode-array Detector
Signal A
254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 20 Hz
Stop time Stop time As pump/No limit
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