2D-LC User Guide 147
6 Method Parameters
Set the 2D-LC Method parameters
Use the Flush Gradient
The Flush Gradient can be used to flush the transfer capillaries and Sample
Loops. You can choose to use the analytical gradient or you can set up a custom
flush gradient. If you choose the Customize flush gradient option, specify a
gradient Duration. The Equilibration time is the same as that set in the Gradient
Phase section.
1 To use the analytical gradient as flush gradient, select the radio button Use
analytical gradient as flush gradient.
OR
To customize a flush gradient, select the radio button Customize flush gradient
and use the table to set up the custom gradient:
a Specify the duration time in the Analysis duration field.
The equilibration value is read only and is defined in the Gradient Phase
settings, see “Specify the Gradient Phase” on page 139.
b Specify the time for the change of solvent composition in the Time [min]
field.
c Specify the percentage of solvent that channel B delivers at the specified
time.
Channel A always delivers the remaining volume, %A = (100 - %B). The
solvent composition changes linearly from one setpoint to the next.
Flush Gradient is only be used in heart cutting mode. If you have selected
comprehensive, this feature is unavailable.
Setup Flush Gradient can also be done graphically in the preview. The Flush
Gradient is displayed in orange.
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