2D-LC User Guide 193
8 Run the System
Familiarization to Start a System Run
Familiarization to Start a System Run
The familiarization procedure illustrates the system´s 2D-LC capabilities and
supports the user to start the method for a specific analytical task. The
familiarization procedure will guide the user through the most important setups
and analysis function.
The sample provided with the familiarization procedure can be determined with a
UV-detector and a mass spectrometer. The methods to analyze the starter
sample are delivered together with the full package to ensure a smooth
familiarization and checkout procedure. With the given method, peaks will
overlap in the first dimension and will be separated in the second dimension.
The Agilent 1290 Infinity II 2D-LC Solution is delivered together with all required
parts for a complete familiarization procedure for (multiple) heart-cutting and
comprehensive 2D-LC.
Methods for system preparation and checkout runs are available on the Agilent
2D-LC Software data media for recommended Infinity II system configurations.
Not all possible configurations can be shown here. Therefore, adapt these
methods for other configurations and modules if necessary.
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