2D-LC User Guide 191
7 Method Development of Active Solvent Modulation (ASM)
Optimize Dilution Through Method Settings
Optimize Dilution Through Method Settings
Figure 104 Optimizing separation using a lower percentage of B for the ASM and column
equilibration phase (example)
For optimizing separation, you may use a lower percentage of B for the ASM
phase and column equilibration phase compared to the original gradient for
increasing dilution before the
2
D column.
If for example the original analytical gradient started at 20 % B, you may use an
ASM phase of for example 2 % B for diluting
1
D solvent more strongly during the
ASM phase by changing the gradient start condition and adding a line to the 2D
gradient table for the ASM phase. The starting point for the analytical gradient
does not change. The solvent composition of the equilibration phase is
automatically reduced to the start condition.
Apply high-resolution sampling with small cut sizes. Small cut sizes reduce the
transfer of solvent volume from
1
D to
2
D, which can further improve solvent
compatibility and 2D resolution.
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