2 Concepts of 2D-LC
Heart-Cutting 2D-LC (LC-LC)
2D-LC User Guide 18
Heart-Cutting 2D-LC (LC-LC)
The following items are characteristic for LC-LC:
• Only parts of the effluent of the
1
D column - only the peaks of interest eluted
from the
1
D column - are injected to the
2
D column
• A peak from the first dimension is sampled as a whole and a method with a
lower flow rate and a gradient typically with a longer run time than the
collection time is used to improve separation efficiency
• Typically longer columns with higher separation efficiency are used in
2
D
column
Heart-Cutting 2D-LC (LC-LC) is the method of choice if the samples to analyze
are known or to improve confidence of an experiment (pharma, method
development and so on).
Multiple peaks eluted from the first dimension column can be sampled and
analyzed in the second dimension but the run time of the second dimension
must match the retention time between two first dimension peaks. A started
second dimension analysis will always be finished! Thus, a second peak being
eluted from the first dimension might be lost, if sampled while the second
dimension analysis is still running.
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