2D-LC User Guide 25
2 Concepts of 2D-LC
Multiple Heart-Cutting and High Resolution Sampling 2D-LC
High Resolution Sampling - Peak Parking Principles
In the HiRes sampling mode, the multiple heart-cutting (MHC) valve is switched
before and after parking the peak. This has the following consequences:
• Each loop for consecutive snips stores the same sample volume.
• First and last loop cannot be used for parking.
• Solvent transfer from
1
D to
2
D can be reduced.
• Cut number 5 cannot be parked entirely in the sample loop. Otherwise cut 6
would got partially to the transfer capillary and would therefore be lost or spoil
cut 5.
Cut 5 stays partially in the transfer line and is immediately being analyzed in
2
D.
• For parking cut 6 in the sample loop, the cut first needs to be moved from the
2D-LC valve to the deck valve.
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