2D-LC User Guide 379
14 Theoretical Background
Solvent Elution Modes
Effect of shifted gradient elution mode in the
2
D
Figure 180
2
D gradient mode compared to isocratic mode and its effect on resolution
sg
as achieved in shifted gradient mode is larger than
g
achieved in standard
gradient elution mode. This can lead to an improved peak detection and
improved separation.
See D. Li and O. J. Schmitz “Use of Shift Gradient in the Second Dimension to
Improve the Separation Space in Comprehensive Twodimensional Liquid
Chromatography” Anal. Bioanal. Chem. 405, 6511-6517 (2013)
Resolution optimization
Į
Į
Gradient mode
Shifted gradient mode
g
sg
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