2D-LC User Guide 377
14 Theoretical Background
Solvent Elution Modes
• Advancing isocratic
Nearly isocratic conditions are used in each
2
D separation, with slightly
increasing solvent strength in each successive run.
The
2
D pumping system is fed with a shallow gradient in eluent composition
over the course of the
2
D separation.
Figure 179 Advancing isocratic mode
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