6 Method Parameters
Set the 2D-LC Method parameters
2D-LC User Guide 140
Use Loop Flushing and Active Solvent Modulation (ASM)
If your 2D-LC instrument is equipped with the G4236A 2D-LC ASM Valve, this
method development feature helps finding the optimal dilution of
1
D solvents in
the sample loop. ASM leads to best
2
D resolution at lowest cycle time.
ASM settings of 2D-LC method parameters allow switching on and off the use of
the ASM functionality.
• If this option is off, it works as a standard 2D-LC valve without dilution.
• If this option is on, the user can set how often he wants to flush the sample
loop during the ASM phase.
1 To use ASM, mark the ASM check box.
This action activates the Active Solvent Modulation.
The ASM Factor is a read-only value and cannot be changed.
For visual verification of the ASM phase, you can check the Analytical Gradient
Graph. There you can see the impact of the ASM phase before the
2
D run starts.
The gradient with ASM increases the cycle/modulation time.
To change the ASM factor you must install and configure the new ASM capillary
in the 2D-LC UI, see “2D-LC Capillaries Configuration Tool” on page 283.
There are four different ASM capillaries available. You can find more info for
Installation and Configure of different ASM capillaries for optimizing the results,
see “Connecting the 2D-LC Valve, ASM (G4243A)” on page 70.
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