2D-LC User Guide 141
6 Method Parameters
Set the 2D-LC Method parameters
2 Specify the number of times to flush the sample loop in the Flush Sample Loop
field.
The total flush time is calculated and displayed.
Flushing the sample loop three times is typically enough and the recommended
default. Less time may be sufficient and can be verified during optimization. The
user interface displays how long the flushing will take.
When using the Active Solvent Modulation (ASM), the valve cycle has four
switches - twice as many as for standard 2D-LC valve. More switches per
injection affect the lifetime of the rotor seal and must be respected for
maintenance intervals.
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