6 Method Parameters
Set the 2D-LC Method parameters
2D-LC User Guide 142
Modulation
The Modulation section shows the 2D-LC Cycle/Modulation time, which is the
sum of the analysis time and the equilibration time specified in the Gradient
Phases section. The modulation time also depends on the sample loop built into
the instrument, see “Recommendations for Instrument Setup” on page 60. These
values are read-only and cannot be edited.
Heart cutting
Cyle/Modulation time The cycle time reflects the duration of an LC-LC injection cycle in the second
dimension. After that time, the solvent composition gradient for the next cut
will be repeated.
Loop volume The Loop volume represents the configured sample loop volume.
The info of the loop filling in heart cutting is displayed in the sampling table.
Comprehensive
Cycle/Modulation time The Modulation time reflects the duration of one LCxLC injection cycle in the
second dimension. After that time, the solvent composition gradient will be
repeated.
Loop volume Loop volume represents the configured sample loop volume.
Loop filling Loop filling represents the actual loop filling value.
If the loop filling for LCxLC is smaller than 20 % or higher than 80 %, a notification
triangle will be displayed.
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